A Quantitative Luminol-Based Assay for ROS Burst Detection in Potato Leaves in Response to Biotic Stimuli

Muhammad Awais Zahid; Ramesh R Vetukuri; Erik Andreasson

doi:10.1007/978-1-0716-2517-0_22

A Quantitative Luminol-Based Assay for ROS Burst Detection in Potato Leaves in Response to Biotic Stimuli

Methods Mol Biol. 2022:2536:395-402. doi: 10.1007/978-1-0716-2517-0_22.

Authors

Muhammad Awais Zahid¹, Ramesh R Vetukuri², Erik Andreasson³

Affiliations

¹ Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Alnarp, Sweden. muhammad.awais.zahid@slu.se.
² Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.
³ Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Alnarp, Sweden.

PMID: 35819616
DOI: 10.1007/978-1-0716-2517-0_22

Abstract

Reactive oxygen species (ROS) are important signaling agents in plants and animals. They are involved in diverse processes, including activation of immune responses to pathogen infection. Biphasic detection of ROS in response to pathogen perception is becoming more popular even in important crops like potato as means of screening different germ plasms and mutants generated by for example CRISPR-Cas9 as well as identifying signaling pathways. Here we describe a detailed protocol for quantifying ROS bursts induced in potato leaf discs in response to a bacterial elicitor and Phytophthora infestans.

Keywords: Immunity; Luminol; Oxidative burst; Potato; ROS; Reactive oxygen species; flg22.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Luminol / metabolism
Phytophthora infestans*
Plant Leaves / metabolism
Reactive Oxygen Species / metabolism
Solanum tuberosum* / metabolism

Substances

Reactive Oxygen Species
Luminol