Pseudomonas boanensis sp. nov., a bacterium isolated from river water used for household purposes in Boane District, Mozambique

Abstract

A Gram-negative rod with a single polar flagellum was isolated from a freshwater reservoir used for household purposes in Boane District, near Maputo, Mozambique, and designated as strain DB1^T. Growth was observed at 30-42 °C (optimum, 30-37 °C) and with 0.5-1.5 % NaCl. Whole-genome-, rpoD- and 16S rRNA-based phylogenies revealed this isolate to be distant from other Pseudomonas species with Pseudomonas resinovorans, Pseudomonas furukawaii and Pseudomonas lalkuanensis being the closest relatives. Phenotypic analyses of strain DB1^T showed marked differences with respect to type strains P. resinovorans CCUG 2473^T, P. lalkuanensis CCUG 73691^T, P. furukawaii CCUG 75672^T and Pseudomonas otiditis CCUG 55592^T. Taken together, our results indicate that strain DB1^T is a representative of a novel species within the genus Pseudomonas for which the name Pseudomonas boanensis is proposed. The type strain is DB1^T (=CCUG 62977^T=CECT 30359^T).

Keywords: Mozambique; Pseudomonas; Pseudomonas boanensis; novel species; river water.