Anisakis spp, DNA detection in paraffin-embedded tissue biopsies recovered from patients with gastritis using real-time PCR in Bushehr, Persian Gulf, Iran

Mol Biochem Parasitol. 2022 Sep:251:111494. doi: 10.1016/j.molbiopara.2022.111494. Epub 2022 Jul 8.

Abstract

Anisakiasis is a zoonotic fish-born parasitic disease caused by anisakid nematodes. Paraffin-embedded blocks containing biopsy samples taken from patients suffering gastritis with unknown causes were investigated by real-time PCR, in the Bushehr region, Iran; where human anisakiasis has not been reported, so far. A total of 50 paraffin-embedded blocks were randomly selected from 250 archived blocks of the patients with gastritis. A SYBER green-based real-time PCR targeting the ITS1 region was developed for the identification of Anisakis genus. An 86 bp partial fragment of the Anisakis spp. ITS1 gene was amplified successfully. A total of 3 out of 50 samples (6 %) had positive amplification in the samples and their pathology reports showed a significant finding of moderate chronic gastritis with or without ulcers. In conclusion, the developed qPCR could be used for detecting Anisakis spp. larval DNA in human biopsy blocks. This study showed the hidden human cases of anisakiasis in the Bushehr for the first time.

Keywords: Anisakiasis; Biopsy; Iran; Persian Gulf; Real time PCR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anisakiasis* / diagnosis
  • Anisakiasis* / parasitology
  • Anisakiasis* / veterinary
  • Anisakis* / genetics
  • Biopsy
  • DNA, Ribosomal Spacer / genetics
  • Fish Diseases* / diagnosis
  • Fish Diseases* / parasitology
  • Gastritis* / diagnosis
  • Humans
  • Indian Ocean
  • Iran
  • Larva / genetics
  • Paraffin Embedding
  • Real-Time Polymerase Chain Reaction
  • Zoonoses / parasitology

Substances

  • DNA, Ribosomal Spacer