Trophic transfer and environmental safety of carbon dots from microalgae to Daphnia

Abstract

The application of carbon dots (CDs), a novel carbon nanomaterial, is extensive, leading to inevitable CD pollution. However, studies on their environmental fate and related risks to aquatic ecosystems are limited. Here, the trophic transfer of CDs from Chlorella pyrenoidosa to Daphnia magna and their toxic effects on the two organisms were analyzed. ¹⁴C-labelling was used to quantify and evaluate the fate of CDs. The results showed that the radioactivity of CDs in water was >80 % of the initial radioactivity, and that water extractable residues were dominant in organisms, with only 3 % or less recovered from the mineralization product ¹⁴CO₂. The distribution of radioactivity illustrated how the exposure routes changed the fate of CDs in aquatic environments. CD aggregates were found in algal cells and Daphnia intestinal tract, indicating the cellular uptake of CDs in these aquatic organisms. Wall-membrane detachment, cell collapse, and rupture were observed in the ultrastructural investigations of microalgae, whereas pneumatosis cystoides intestinalis was observed in the ultrastructural investigations of D. magna. CD exposure affected the growth and chlorophyll content of C. pyrenoidosa as well as the feeding behavior, oxidative stress system, digestive system, and symbiotic bacteria of D. magna. The toxicity of CDs is also affected by the route of exposure. These findings suggest that dietary exposure to CDs was more likely to cause environmental risk and adverse effects than aqueous exposure, and the environmental risks associated with CDs should not be underestimated.

Keywords: Aquatic ecosystem; Biological responses; Carbon dots; Cellular uptake; Environment fate and safety.