Fluid-driven bacterial accumulation in proximity of laser-textured surfaces

Abstract

In this work we investigated the role of fluid in the initial phase of bacterial adhesion on textured surfaces, focusing onto the approach of the bacterial cells towards the surface. In particular, stainless steel surfaces textured via femtosecond laser interaction have been considered. The method combined a simulation routine, based on the numerical solution of Navier-Stokes equations, and the use of a theoretical model, based on the Smoluchowski's equation. Results highlighted a slowdown of the fluid velocity field in correspondence of the surface dales. In addition, a shear induced accumulation on the top of the surface protrusions was predicted for motile bacterial species, E. coli. In particular, we observed a role of the surface protrusions in increasing the range over which motile bacterial species are attracted towards the surface through a rheotactic mechanism. In other words, we found that, in certain conditions of fluid flow and textured surface morphology, surface protrusions act as a sort of "rheotactic antennas".

Keywords: Bacteria; Laser texturing; Rheotaxis.