Renoprotective and Cardioprotective Potential of Moricandia sinaica (Boiss.) against Carbon Tetrachloride-Induced Toxicity in Rats

Abdelaaty A Shahat; Heba D Hassanein; Riaz Ullah; Ali S Alqahtani; Husseiny A Husseiny; Alicja Kowalczyk; Abdel-Razik H Farrag

doi:10.1155/2022/8545695

Renoprotective and Cardioprotective Potential of Moricandia sinaica (Boiss.) against Carbon Tetrachloride-Induced Toxicity in Rats

Evid Based Complement Alternat Med. 2022 Jun 29:2022:8545695. doi: 10.1155/2022/8545695. eCollection 2022.

Authors

Abdelaaty A Shahat^{1

2}, Heba D Hassanein², Riaz Ullah¹, Ali S Alqahtani¹, Husseiny A Husseiny³, Alicja Kowalczyk⁴, Abdel-Razik H Farrag⁵

Affiliations

¹ Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
² Chemistry of Medicinal Plants Department, National Research Centre, 33 El Bohouth St. (Former El Tahrirst.), Dokki, P.O. 12622, Giza, Egypt.
³ Faculty of Pharmacy, Misr University for Science and Technology (MUST), Giza, Egypt.
⁴ Department of Environmental Hygiene and Animal Welfare, Wrocław University of Environmental and Life Sciences, Chełmońskiego 38C, Wrocław 51-630, Poland.
⁵ Pathology Department, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), Dokki, P.O. 12622, Giza, Egypt.

Abstract

The goal of the current study was to assess the nephroprotective and cardioprotective potential of Moricandia sinaica methanol extract (MOR-1), as well as its butanol (MOR-2) and aqueous (MOR-3) fractions against carbon tetrachloride (CCl₄)-induced nephro and cardio-toxicity. Cardiac function was assessed using the biochemical parameters lactate dehydrogenase (LDH) and creatinine kinase (CK). Renal function was examined using the biochemical parameters creatinine and uric acid. The levels of nonprotein sulfhydryls (NPSH) and malondialdehyde (MDA) were used as markers of oxidative strain. A dose of 100 and 200 mg/kg of butanol fraction given prior to CCl₄ treatment significantly (p < 0.05 - 0.001) protected against elevated LDH and CK levels. Similarly, treatment with silymarin (10 mg/kg) and butanol fraction (100 and 200 mg/kg) significantly (p < 0.05 - 0.001) boosted total protein levels compared to CCl₄ treatment alone. The silymarin (10 mg/kg) and butanol fraction (100 and 200 mg/kg) also provided a significant (p < 0.05 - 0.001) protective effect for MDA levels. Methanol extract (MOR-1) and butanol (MOR-2) showed significant results and were recommended for further pharmacological and screening for active constituents.