Gingiva-Derived Mesenchymal Stem Cells Attenuate Imiquimod- (IMQ-) Induced Murine Psoriasis-Like Skin Inflammation

Ziyu Ye; Yanfang Liang; Bihua Lin; Yanyun Li; Xingxing Chai; Jiachun Lian; Xueying Zhang; Zhengping Che; Jincheng Zeng

doi:10.1155/2022/6544514

Gingiva-Derived Mesenchymal Stem Cells Attenuate Imiquimod- (IMQ-) Induced Murine Psoriasis-Like Skin Inflammation

Stem Cells Int. 2022 Jun 30:2022:6544514. doi: 10.1155/2022/6544514. eCollection 2022.

Authors

Ziyu Ye^{1

2}, Yanfang Liang³, Bihua Lin^{1

4

5}, Yanyun Li¹, Xingxing Chai¹, Jiachun Lian^{1

6}, Xueying Zhang^{1

6}, Zhengping Che^{1

6}, Jincheng Zeng^{1

2

4

5}

Affiliations

¹ Dongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Dongguan 523808, China.
² Guangdong Xinghai Institute of Cell, Dongguan 523808, China.
³ Department of Pathology, Dongguan Hospital Affiliated to Jinan University, Binhaiwan Central Hospital of Dongguan, Dongguan 523000, China.
⁴ Key Laboratory of Medical Bioactive Molecular Research for Department of Education of Guangdong Province, School of Basic Medicine, Guangdong Medical University, Dongguan 523808, China.
⁵ Collaborative Innovation Center for Antitumor Active Substance Research and Development, Department of Biochemistry and Molecular Biology, School of Basic Medicine, Guangdong Medical University, Zhanjiang 524023, China.
⁶ Institute of Laboratory Medicine, School of Medical Technology, Guangdong Medical University, Dongguan 523808, China.

Abstract

Human gingiva-derived mesenchymal stem cells (GMSCs) are isolated from the gingival propria with promising regenerative, immunomodulatory, and anti-inflammatory properties. Recently, several studies, including ours, have found that GMSCs have the therapeutic potentials of nerve regeneration and skin disorders in various types such as the cell itself, cell-free conditioned medium, or extracellular vesicles (EVs). However, the mechanobiological behavior of GMSCs is closely related to the culture conditions. Therefore, the purpose of this study was to evaluate the function of human GMSCs on imiquimod- (IMQ-) induced murine psoriasis-like skin inflammation in two-dimensional (2D) and three-dimensional (3D) culture conditions. Here, we isolated and characterized GMSCs in 2D and 3D culture conditions and found that GMSCs in 2D and 3D infusion can significantly ameliorate the IMQ-induced murine psoriasis-like skin inflammation, reduce the levels of Th1- and Th17-related cytokines IFN-γ, TNF-α, IL-6, IL-17A, IL-17F, IL-21, and IL-22, and upregulate the percentage of spleen CD25⁺CD3⁺ T cells while downregulate the percentage of spleen IL-17⁺CD3⁺ T cells. In summary, our novel findings reveal that GMSCs in 2D and 3D infusion may possess therapeutic effects in the treatment of psoriasis.