Genkwanin Prevents Lipopolysaccharide-Induced Inflammatory Bone Destruction and Ovariectomy-Induced Bone Loss

Xin Fu; Xiaochen Sun; Chenxi Zhang; Nanning Lv; Huan Guo; Chunlei Xing; Juan Lv; Jiwen Wu; Xiaoli Zhu; Mingming Liu; Li Su

doi:10.3389/fnut.2022.921037

Genkwanin Prevents Lipopolysaccharide-Induced Inflammatory Bone Destruction and Ovariectomy-Induced Bone Loss

Front Nutr. 2022 Jun 23:9:921037. doi: 10.3389/fnut.2022.921037. eCollection 2022.

Authors

Xin Fu^{1

2}, Xiaochen Sun³, Chenxi Zhang¹, Nanning Lv^{4

5}, Huan Guo¹, Chunlei Xing¹, Juan Lv¹, Jiwen Wu², Xiaoli Zhu², Mingming Liu^{4

5}, Li Su¹

Affiliations

¹ Institute of Translational Medicine, Shanghai University, Shanghai, China.
² Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai, China.
³ School of Medicine, Shanghai University, Shanghai, China.
⁴ Lianyungang Second People's Hospital, Lianyungang, China.
⁵ Lianyungang Clinical School of Xuzhou Medical University, Lianyungang, China.

Abstract

Objectives: The first objective of this study was to probe the effects of genkwanin (GKA) on osteoclast. The second goal of this study was to study whether GKA can protect lipopolysaccharide (LPS) and ovariectomized (OVX) induced bone loss.

Materials and methods: Various concentrations of GKA (1 and 10 mg/kg) were injected into mice. Different concentrations of GKA (1 and 5 μM) were used to detect the effects of GKA on osteoclast and osteoblast.

Key findings: GKA attenuated the osteoclast differentiation promoted by RANKL and expression of marker genes containing c-fos, ctsk as well as bone resorption related gene Trap and to the suppression of MAPK signaling pathway. In addition, GKA induced BMMs cell apoptosis in vitro. Moreover, GKA prevented LPS-induced and ovariectomized-induced bone loss in mice.

Conclusion: Our research revealed that GKA had a potential to be an effective therapeutic agent for osteoclast-mediated osteoporosis.

Keywords: MAPK; genkwanin; osteoclast; ovariectomy; postmenopausal osteoporosis.