Geographically diverse lineage of mycobacterium tuberculosis in Sub-Himalayan region of Kangra, Himachal Pradesh

S Thakur; V Chauhan; S C Jaryal; C Goel

doi:10.1016/j.ijmmb.2022.06.008

Geographically diverse lineage of mycobacterium tuberculosis in Sub-Himalayan region of Kangra, Himachal Pradesh

Indian J Med Microbiol. 2022 Oct-Dec;40(4):588-589. doi: 10.1016/j.ijmmb.2022.06.008. Epub 2022 Jul 8.

Authors

S Thakur¹, V Chauhan², S C Jaryal³, C Goel⁴

Affiliations

¹ IGMC Shimla, HP, India.
² IGMC Shimla, HP, India. Electronic address: drvivekshimla@yahoo.com.
³ Dr. RPGMC Kangra, HP, India.
⁴ ICMR Model Rural Health Research Unit, Una, HP, India.

PMID: 35811260
DOI: 10.1016/j.ijmmb.2022.06.008

Abstract

We present the lineages of Mycobacterium tuberculosis (MTB) found using spoligotyping in the Sub-Himalayan Region of Kangra District in Himachal Pradesh. The DNA from clinical isolates was used for spoligotyping using a PCR based method to simultaneously detect and type Mycobacterium tuberculosis complex, performed by an outsource agency namely Mapmygenome, Hyderabad. Spoligotyping and database comparison was done. We found the following families: Indo Oceanic (51.2%) Central Asian (37.8%), West African (4.8%), East Asian (3.6%), Euro American and M.africanum (1.2%) each. There were genetically diverse strains of MTB causing pulmonary tuberculosis in Kangra region, indicating cosmopolitan nature of its population.

MeSH terms

Genotype
Humans
Mycobacterium tuberculosis* / genetics
Polymerase Chain Reaction
Tuberculosis, Pulmonary* / diagnosis
Tuberculosis, Pulmonary* / epidemiology
Tuberculosis, Pulmonary* / microbiology