Effect of Iron Deposition on Native T1 Mapping and Blood Oxygen Level Dependent for the Assessment of Liver Fibrosis in Rabbits With Carbon Tetrachloride Intoxication

Abstract

Rationale and objectives: This study aimed to explore the effect of iron deposition on native T1 mapping and blood oxygen level-dependent (BOLD) imaging in detecting liver fibrosis (LF) in a rabbit model.

Materials and methods: An LF group (n = 100) was established by subcutaneously injecting 50% carbon tetrachloride (CCl₄) oil solution, and 20 normal rabbits composed a control group. Native T1 mapping and BOLD were performed, and the T1_native and R₂* quantitative parameters were analyzed by receiver operating characteristic (ROC) and multiple logistic regression analyses, with histopathological results and liver iron content (LIC) serving as reference standards.

Results: In total, 18, 17, 16, 18, and 15 rabbits were histopathologically diagnosed with LF stages F0, F1, F2, F3, and F4, respectively. T1_native (r = 0.47), R₂* (r = 0.75) and LIC (r = 0.61) increased with LF stage progression (p < 0.001). Compared to T1_native values, R₂* performed better in diagnosing the LF stage, especially for distinguishing F1-F2 from F3-F4 (AUC = 0.66 vs. 0.91, p = 0.01). Combined with the LIC, both T1_native and R₂* showed improved diagnostic value in comparison to the individual imaging techniques, particularly for diagnosing F0 vs. F1-F2 and F0 vs. F1-F4, with AUC values of 0.90 vs. 0.70 (p = 0.01) and 0.93 vs. 0.77 (p = 0.01) for T1_native + LIC vs. LIC, respectively.

Conclusion: BOLD imaging performed better than native T1 mapping in predicting and diagnosing LF stage progression. The decrease in diagnostic accuracy caused by the deposition of liver iron is a potential pitfall in the assessment of LF with BOLD imaging and native T1 mapping.

Keywords: blood oxygen level-dependent (BOLD) imaging; iron deposition; liver fibrosis; native T1 mapping.