Preparation of cellulose-based chromatographic medium for biological separation: A review

Tian Yao; Jialing Song; Ya Gan; Liangzhi Qiao; Kaifeng Du

doi:10.1016/j.chroma.2022.463297

Preparation of cellulose-based chromatographic medium for biological separation: A review

J Chromatogr A. 2022 Aug 16:1677:463297. doi: 10.1016/j.chroma.2022.463297. Epub 2022 Jul 2.

Authors

Tian Yao¹, Jialing Song², Ya Gan², Liangzhi Qiao³, Kaifeng Du⁴

Affiliations

¹ Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering and Technology Research Center of Coarse Cereal Industralizationa, School of Food and Biological Engineering, Chengdu University, Chengdu 610106, PR China. Electronic address: 13982152430@163.com.
² Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering and Technology Research Center of Coarse Cereal Industralizationa, School of Food and Biological Engineering, Chengdu University, Chengdu 610106, PR China.
³ Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, PR China.
⁴ Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, PR China. Electronic address: kfdu@scu.edu.cn.

PMID: 35809519
DOI: 10.1016/j.chroma.2022.463297

Abstract

Cellulose is a kind of renewable linear polysaccharide with good safety, hydrophilicity, biocompatibility and biodegradability and has become a commonly used chromatographic medium for biological separation and purification. The purpose of this paper is to describe the recent developments in the applications of cellulose-based absorbents as chromatographic medium. This review also attempts to explain the current situation of cellulose-based chromatographic medium from the aspects of cellulose dissolution, common strategies for generating spherical particles of cellulose and its derivatives (such as titration, emulsification, microfluidic and other synthesis methods) and improvement of adsorption properties. Furthermore, non-spherical cellulose-based chromatographic medium are also described briefly.

Keywords: Cellulose; Chromatographic medium; Emulsification; Microspheres.

Publication types

Review

MeSH terms

Adsorption
Cellulose* / chemistry
Chromatography*
Hydrophobic and Hydrophilic Interactions

Substances

Cellulose