Lysosome-targetable brightly green fluorescence carbon dots for real-time monitoring in cell and highly efficient removal in environment of hypochlorite

Abstract

Due to the lacks of lysosome localization group and reaction/interaction site for hypochlorite (ClO^-) on the surface of the carbon dots (C-dots), no C-dots-based lysosome-targeted fluorescence probes have, so far, been reported for real-time monitoring intracellular ClO^-. In this work, 1,3,6-trinitropyrene (TNP) was used as a precursor to prepare C-dots with maximum excitation and emission wavelengths at 485 and 532 nm, respectively, and quantum yield ∼ 27% by a hydrothermal approach at 196 °C for 6 h under a reductive atmosphere. The brightly green C-dots can sensitively and quickly respond to ClO^- in aqueous solution through surface chemical reaction, showing a linear relationship in the range of 0.5-120 μΜ ClO^- with 0.27 μΜ of limit of detection (LOD). Most significantly, the C-dots can localize at intracellular lysosome to image ClO^- in lysosomes. Also, the magnetic nanocomposites (C-dots@Fe₃O₄ MNCs) were fabricated via a simple electrostatic self-assembly between Fe₃O₄ magnetic nanoparticles (Fe₃O₄ MNPs) and C-dots for highly efficient removal of ClO^- in real samples. Therefore, lysosome-targetable C-dots-based probes for real-time monitoring ClO^- were successfully constructed, opening up a promising door to investigate the biological functions and pathological roles of ClO^- at organelle levels.

Keywords: Carbon dots (C-dots); Fe(3)O(4) magnetic nanoparticles (Fe(3)O(4) MNPs); Fluorescence probe; Hypochlorite (ClO(-)); Lysosome localization.