Comparative LC-LTQ-MS-MS Analysis of the Leaf Extracts of Lantana camara and Lantana montevidensis Growing in Egypt with Insights into Their Antioxidant, Anti-Inflammatory, and Cytotoxic Activities

Mariam I Gamal El-Din; Nouran M Fahmy; Fulin Wu; Maha M Salem; Omar M Khattab; Hesham R El-Seedi; Michal Korinek; Tsong-Long Hwang; Ahmed K Osman; Mohamed El-Shazly; Shaimaa Fayez

doi:10.3390/plants11131699

Comparative LC-LTQ-MS-MS Analysis of the Leaf Extracts of Lantana camara and Lantana montevidensis Growing in Egypt with Insights into Their Antioxidant, Anti-Inflammatory, and Cytotoxic Activities

Plants (Basel). 2022 Jun 27;11(13):1699. doi: 10.3390/plants11131699.

Authors

Mariam I Gamal El-Din¹, Nouran M Fahmy¹, Fulin Wu^{2

3}, Maha M Salem⁴, Omar M Khattab^{2

5}, Hesham R El-Seedi^{2

5

6

7}, Michal Korinek^{8

9}, Tsong-Long Hwang^{9

10

11

12}, Ahmed K Osman¹³, Mohamed El-Shazly^{1

14}, Shaimaa Fayez¹

Affiliations

¹ Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo 11566, Egypt.
² Pharmacognosy Group, Department of Pharmaceutical Biosciences, Biomedical Centre, Uppsala University, P.O. Box 591, 751 24 Uppsala, Sweden.
³ School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
⁴ Biochemistry Division, Chemistry Department, Faculty of Science, Tanta University, Tanta 31527, Egypt.
⁵ Department of Chemistry, Faculty of Science, Menoufia University, Shebin El-Kom 32512, Egypt.
⁶ International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang 212013, China.
⁷ International Joint Research Laboratory of Intelligent Agriculture and Agri-Products Processing, Jiangsu Education Department, Jiangsu University, Zhenjiang 212013, China.
⁸ Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
⁹ Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Kweishan, Taoyuan 33302, Taiwan.
¹⁰ Research Center for Chinese Herbal Medicine, Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Kweishan, Taoyuan 33302, Taiwan.
¹¹ Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan.
¹² Department of Chemical Engineering, Ming Chi University of Technology, New Taipei City 243303, Taiwan.
¹³ Department of Botany and Microbiology, Faculty of Science, South Valley University, Qena 83523, Egypt.
¹⁴ Department of Pharmaceutical Biology, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo 11835, Egypt.

Abstract

Lantana camara L. and Lantana montevidensis Briq. (F. Verbenaceae) are invasive ornamental weeds native to the tropical regions of Africa and America. The leaves of both species have been traditionally used as infusions for treating fever, rheumatism, and cancer. LC-MS-MS-guided profiling of the methanolic extracts of the leaves of L. camara and L. montevidensis growing in Egypt led to the putative identification of 59 compounds belonging to terpenoids, flavonoids, iridoid glycosides, phenolic acids, and their derivatives. The in-vitro antioxidants and anti-inflammatory and anticancer activities of the two extracts were investigated. L. camara and L. montevidensis inhibited DPPH^• (IC₅₀ = 34.01 ± 1.32 and 47.43 ± 1.74 µg/mL), ABTS⁺ (IC₅₀ = 30.73 ± 1.42 and 40.37 ± 1.51 µg/mL), and superoxide anion (IC₅₀ = 1.57 ± 0.19 and 1.31 ± 0.14 μg/mL) free radicals. A potent anti-inflammatory effect was observed for both species through the inhibition of elastase release in fMLF/CB-induced human neutrophils (IC₅₀ = 2.40 ± 0.16 and 1.90 ± 0.07 μg/mL). The extracts showed significant cytotoxic activity against a panel of cancer cell lines with the most potent activity against Caco cells (IC₅₀ = 45.65 ± 1.64 and 40.67 ± 1.52 µg/mL for L. camara and L. montevidensis, respectively). Western blotting supported by FACS analysis revealed that the extracts inhibited cancer cell proliferation, reduced metastasis, and induced apoptosis resulting in cell cycle arrest. This was achieved via increasing mRNA and protein expressions of p53 and GSK-3β as well as decreasing the expression of PI3K, Akt, and cyclin D1.

Keywords: LC–MS–MS; Lantana camara; Lantana montevidensis; anti-inflammatory; antioxidant; cytotoxicity.

Grants and funding

This study was supported by grants from the Ministry of Science and Technology (MOST 111-2321-B-182-001, MOST 109-2320-B-650-001-MY3, MOST 108-2320-B-255-003-MY3, and MOST 109-2327-B-255-001), Chang Gung University of Science and Technology (ZRRPF3L0091), and Chang Gung Memorial Hospital (CMRPF1J0051~3, CORPF1L0011, CMRPF1L0071, and BMRP450).