While there are many studies that have reported methods for tracing the geographical origin of seafoods, most of them have focused on identifying parameters that can be used effectively and not the direct application of these methods. In this study, we attempted to differentiate the geographical origins of the Manila clam R. philippinarum collected from different sites in Korea, the Democratic People's Republic of Korea, and China using a combination of analyses based on dual-element isotopes, fatty acids (FAs), and compound-specific isotopic analysis of FAs. We hypothesized that a stepwise application of new parameters to unclassified samples could achieve this objective by integrating new information while reducing time and labor. The FA profiles and compound-specific carbon isotopic values of FAs were found to enhance the discrimination power of determining the geographic origin up to 100%. Our findings demonstrate the advantageousness of using several parameters simultaneously over the conventional method of employing individual analytical methods when identifying geographic origins of the Manila clam, which could have implications for tracing the origins of different shellfish species or other food products as well.
Keywords: authentication; compound-specific isotope analysis; dual-isotopes; fatty acids; linear discriminant analysis; manila clam.