The complete chloroplast genome sequence of Heracleum millefolium Diels (Apiaceae)

Dunzhu Ciren; Chen Fan; Xiang Liu; Zhiwei Zhang; Jiashui Wang; Lan Cao

doi:10.1080/23802359.2022.2086073

The complete chloroplast genome sequence of Heracleum millefolium Diels (Apiaceae)

Mitochondrial DNA B Resour. 2022 Jun 30;7(6):1058-1060. doi: 10.1080/23802359.2022.2086073. eCollection 2022.

Authors

Dunzhu Ciren¹, Chen Fan², Xiang Liu³, Zhiwei Zhang³, Jiashui Wang⁴, Lan Cao⁴

Affiliations

¹ University of Tibetan Medicine, Lhasa, China.
² School of Medicine and Chemical Engineering, Yangling Vocational & Technical College, Xianyang, Shsaanxi, China.
³ Chongqing Academy of Chinese Materia Medica, Chongqing, China.
⁴ Research Center for Traditional Chinese Medicine Resources and Ethnic Minority Medicine, Jiangxi university of Chinese Medicine, Nanchang, China.

Abstract

We assembled the complete chloroplast genome of Heracleum millefolium which is a traditional widely used medicinal plant in China. The whole genome is 150,025 bp in length which was divided into four subregions: a large single-copy region (93,645 bp), a pair of 19,458 bp inverted repeats regions, and a small single-copy region (17,464 bp), respectively. Additionally, the chloroplast genome of H. millefolium detected 128 genes, including 85 protein coding genes, 36 transfer RNAs, and eight ribosomal RNAs. The overall GC content of this chloroplast genome is 37.5% and the mean coverage value is 1752.4x. Phylogenetic analysis based on 17 chloroplast genomes dataset was conducted to clarify the relationships of the major clades in Apiaceae. The results strongly supported the monophyly of Heracleum and the closer relationship of H. millefolium and H. candicans.

Keywords: Plastome; medicinal plant; phylogeny; Heracleum.

Grants and funding

This work was supported by the Traditional Tibetan Medicine Administration Bureau under Grant number JJKT2018004; and the University of Tibetan Medicine under Grant number BSDJS-19-13.