PIWI-Directed DNA Elimination for Tetrahymena Genetics

Salman Shehzada; Kazufumi Mochizuki

doi:10.1007/978-1-0716-2380-0_3

PIWI-Directed DNA Elimination for Tetrahymena Genetics

Methods Mol Biol. 2022:2509:53-68. doi: 10.1007/978-1-0716-2380-0_3.

Authors

Salman Shehzada¹, Kazufumi Mochizuki²

Affiliations

¹ Institute of Human Genetics (IGH), CNRS and University of Montpellier, Montpellier, France.
² Institute of Human Genetics (IGH), CNRS and University of Montpellier, Montpellier, France. kazufumi.mochizuki@igh.cnrs.fr.

PMID: 35796956
DOI: 10.1007/978-1-0716-2380-0_3

Abstract

Piwi-bound small RNAs induce programmed DNA elimination in the ciliated protozoan Tetrahymena. Using the phenomenon called codeletion, this process can be reprogrammed to induce ectopic DNA elimination at basically any given genomic location. Here, we describe the usage of codeletion for genetic studies in Tetrahymena and for investigations of the molecular mechanism of Piwi-directed programmed DNA elimination.

Keywords: DNA elimination; Gene knockout; PIWI; Small RNA; Tetrahymena.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Protozoan / genetics
RNA
RNA Interference
RNA, Protozoan / genetics
Tetrahymena* / genetics

Substances

DNA, Protozoan
RNA, Protozoan
RNA