Goji Ferment Ameliorated Acetaminophen-Induced Liver Injury in vitro and in vivo

Chih-Min Yang; Mei-Yin Chien; Li-Yu Wang; Cheng-Hung Chuang; Chao-Hsiang Chen

doi:10.1007/s12602-022-09956-y

Goji Ferment Ameliorated Acetaminophen-Induced Liver Injury in vitro and in vivo

Probiotics Antimicrob Proteins. 2023 Oct;15(5):1102-1112. doi: 10.1007/s12602-022-09956-y. Epub 2022 Jul 7.

Authors

Chih-Min Yang¹, Mei-Yin Chien¹, Li-Yu Wang^{2

3}, Cheng-Hung Chuang⁴, Chao-Hsiang Chen^{5

6}

Affiliations

¹ Ko Da Pharmaceutical Co. Ltd, Pingzhen Dist, No.20-1, Gongye 3rd Rd, Taoyuan, Taiwan.
² Department of Nutrition, Master Program of Biomedical Nutrition, Hungkuang University, No. 1018 Sec. 6 Taiwan Boulevard, Taichung, 43302, Taiwan.
³ Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan.
⁴ Department of Nutrition, Master Program of Biomedical Nutrition, Hungkuang University, No. 1018 Sec. 6 Taiwan Boulevard, Taichung, 43302, Taiwan. chchuang@hk.edu.tw.
⁵ Ko Da Pharmaceutical Co. Ltd, Pingzhen Dist, No.20-1, Gongye 3rd Rd, Taoyuan, Taiwan. cmy@koda.com.tw.
⁶ Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan. cmy@koda.com.tw.

PMID: 35796949
DOI: 10.1007/s12602-022-09956-y

Abstract

This study aimed to investigate the hepatoprotective effects of lyophilized powder of goji ferment (LPGF) against acetaminophen (APAP)-induced hepatic damage in Hep3B cells and in mice. Eleven strains of lactic acid bacteria (LAB) were selected and their hepatoprotection against APAP-induced cellular damage in Hep3B cell line was evaluated. Four strains of LAB, including BCRC11652 (Leuconostoc mesenteroides subsp. mesenteroides), BCRC14619 (Lactobacillus gasseri), KODA-1 (Pediococcus acidilactici), and KODA-2 (Limosilactobacillus fermentum), have hepatoprotective potential against APAP in vitro. Goji significantly stimulated the growth of individual and combined strains of LAB and the optimal fermented condition was the treatment of goji at 10% (w/w) for 24 h. The prepared lyophilized powder of goji ferment (LPGF) containing fifteen combinations of LAB strains was used to explore their hepatoprotection in vitro. LPGF containing all combinations of LAB strains, except for KODA-2, significantly restored APAP-reduced cell viability and improved APAP-increased cellular levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). In mice model, LPGF containing BCRC11652, BCRC14619, and KODA-2 was chosen to evaluate its hepatoprotection against APAP-induced liver injury. LPGF at diverse doses have a tendency but no significant improvement on APAP-reduced body weight gain and liver weight. LPGF significantly decreased APAP-increased serum ALT and AST levels in a dose-dependent manner. At the end of experiment, LPGF significantly and dose-dependently reversed APAP-reduced activities of GSH and antioxidant enzymes, including glutathione peroxidase, superoxide dismutase, and catalase in hepatic tissue. Overall, LPGF was demonstrated to exhibit hepatoprotection against APAP-induced liver injury in vitro and in vivo.

Keywords: Acetaminophen; Goji; Hepatoprotection; Lactic acid bacteria; Lyophilized powder of goji ferment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetaminophen* / adverse effects
Animals
Antioxidants / metabolism
Antioxidants / pharmacology
Chemical and Drug Induced Liver Injury, Chronic*
Glutathione / metabolism
Mice
Oxidative Stress
Powders / pharmacology

Substances

Acetaminophen
Powders
Glutathione
Antioxidants