Evolution of a novel female reproductive strategy in Drosophila melanogaster populations subjected to long-term protein restriction

Abstract

Reproductive output is often constrained by availability of macronutrients, especially protein. Long-term protein restriction, therefore, is expected to select for traits maximizing reproduction even under nutritional challenge. We subjected four replicate populations of Drosophila melanogaster to a complete deprivation of yeast supplement, thereby mimicking a protein-restricted ecology. Following 24 generations, compared to their matched controls, females from experimental populations showed increased reproductive output early in life, both in presence and absence of yeast supplement. The observed increase in reproductive output was without associated alterations in egg size, development time, preadult survivorship, body mass at eclosion, and life span of the females. Further, selection was ineffective on lifelong cumulative fecundity. However, females from experiment regime were found to have a significantly faster rate of reproductive senescence following the attainment of the reproductive peak early in life. Therefore, adaptation to yeast deprivation ecology in our study involved a novel reproductive strategy whereby females attained higher reproductive output early in life followed by faster reproductive aging. To the best of our knowledge, this is one of the cleanest demonstrations of optimization of fitness by fine-tuning of reproductive schedule during adaptation to a prolonged nutritional deprivation.

Keywords: Antagonistic pleiotropy; experimental evolution; fitness trade-offs; nutritional deprivation.