Effects of Nitrogen Addition on Plant Properties and Microbiomes Under High Phosphorus Addition Level in the Alpine Steppe

Junfu Dong; Xiaoyong Cui; Haishan Niu; Jing Zhang; Chuanlu Zhu; Linfeng Li; Zhe Pang; Shiping Wang

doi:10.3389/fpls.2022.894365

Effects of Nitrogen Addition on Plant Properties and Microbiomes Under High Phosphorus Addition Level in the Alpine Steppe

Front Plant Sci. 2022 Jun 20:13:894365. doi: 10.3389/fpls.2022.894365. eCollection 2022.

Authors

Junfu Dong^{1

2}, Xiaoyong Cui², Haishan Niu³, Jing Zhang⁴, Chuanlu Zhu², Linfeng Li², Zhe Pang³, Shiping Wang⁵

Affiliations

¹ Institute of Marine Science and Technology, Shandong University, Qingdao, China.
² College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
³ College of Resource and Environment, University of Chinese Academy of Sciences, Beijing, China.
⁴ College of Grassland Science, Beijing Forestry University, Beijing, China.
⁵ Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, China.

Abstract

Nitrogen (N) addition can increase the vegetative growth, improve the plant production, and restore the degraded terrestrial ecosystems. But, it simultaneously aggravates the soil phosphorus (P) limitation for plant growth, thus affecting its positive effects on ecosystems. However, how plants and soil microorganisms will change under conditions of high P content in soil is still unknown. In this study, we explored the effects of three levels of N addition (0, 7.5, and 15 g.N.m^-2.year^-1) on plants and microorganisms at the high P addition level (13.09 g.P.m^-2.year^-1) in the alpine steppe. We found that the soil microbial community composition had no significant difference between different N addition levels, and the soil AN and AP had a significant effect on the phospholipid fatty acid (PLFA) composition. The abundance of the core PLFAs (i.e., 16:1ω7c, 16:0, a17:1, i17:0, 18:1ω9c, and 18:1ω7c) also remained unchanged after N addition, and microbes at individual, population, and community levels were all correlated with SOM, AK, AN, and pH. Conversely, plant biomass and nutrient content showed linear trends with increasing N addition, especially the dominant functional groups. Specifically, the biomass and plant tissue N content of Gramineae, and the total N content of aboveground biomass were all improved by N addition. They were correlated with soil ammonium and AP. The structural equation modeling (SEM) demonstrated that N addition had a direct negative effect on soil microbial biomass, but an indirect positive effect on aboveground biomass via soil ammonium. These findings clarify the importance of N-amendment in regulating plants and microorganisms under high P conditions and provide a better understanding of the N-added effects in the alpine steppe.

Keywords: nitrogen application; nutrient uptake; plant–microbe interaction; the Qinghai-Tibetan plateau; the core species.