The beneficial effect of moderate wine consumption is attributed to its micronutrients, especially polyphenols and largely depends on the digestion process. This work aimed to examine the influence of in vitro simulated digestion in the presence of complex food matrix on antioxidant and cytotoxic activity of red wine. The obtained results showed that total phenolic content of wine sample after in vitro digestion was higher compared to undigested wine, while the antioxidant activity of these samples was similar before and after digestion. Furthermore, it has been noticed that digested wine showed cytotoxic activity on SKBR3 breast adenocarcinoma cells near 20% after 72 h of treatment. This pioneering study that examined biological potential of in vitro digested wine in the presence of complex food matrix indicate that antioxidant and cytotoxic activity of red wine is preserved after digestion.
Keywords: Red wine; antioxidant activity; cyclic voltammetry; cytotoxic activity; in vitro digestion.