A simple, fast, and efficient method of vortex assisted magnetic dispersive solid phase extraction for separation and pre-concentration of erythromycin in chicken samples prior to high LC-UV determination has been developed. The novel heterogeneous CeZrO2 nanoparticles doped magnetic graphene oxide, for use as an efficient nanosorbent, was synthetised and applied for the adsorption of erythromycin. The synthetised nanosorbent was characterised using both Fourier-transform infra-red (FT-IR) and energy dispersive X-Ray (EDX) spectroscopy together with field emission scanning electron microscopy-EDX. To obtain the best extraction condition and maximum extraction efficiency of erythromycin, the effect of important parameters including pH, amount of sorbent, vortexing time, ionic strength, sample volume, and desorption conditions were investigated. At optimum conditions, a linear range of 0.25-300 µg kg-1, LOD (S/N = 3) of 0.079 µg kg-1, and LOQ (S/N = 10) of 0.270 µg kg-1 were obtained. The precision of the method was established as having an RSD (%) at 100 µg kg-1 of erythromycin for seven replicates of 2.6% and 3.2% for the intra-day and the inter-day, respectively. Recoveries over 94.0% confirmed a high capability of the proposed method for separation and determination of erythromycin residues in chicken being one of the most important animal products.
Keywords: CeZrO2 nanoparticles; Erythromycin; chicken; magnetic dispersive solid phase extraction; magnetic graphene oxide; vortex assisted.