Antibacterial effect of Cu2O/TiO2 photocatalytic composite on Pseudomonas marginalis pv. marginalis

Ying Jiang; Mengna Ding; Lang Liu; Jianguo Cao; Liuhong Chen; Guozheng Huang; Yuning Huo; Xiling Dai

doi:10.1007/s00203-022-03065-8

Antibacterial effect of Cu₂O/TiO₂ photocatalytic composite on Pseudomonas marginalis pv. marginalis

Arch Microbiol. 2022 Jul 6;204(8):462. doi: 10.1007/s00203-022-03065-8.

Authors

Ying Jiang¹, Mengna Ding², Lang Liu¹, Jianguo Cao¹, Liuhong Chen¹, Guozheng Huang³, Yuning Huo⁴, Xiling Dai⁵

Affiliations

¹ College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.
² The Education Ministry Key Lab of Resource Chemistry, Joint International Research Laboratory of Resource Chemistry, Ministry of Education, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Frontiers Science Research Base of Biomimetic Catalysis, and College of Chemistry and Materials Science, Shanghai Frontiers Science Center of Biomimetic Catalysis, Shanghai Normal University, Shanghai, 200234, China.
³ College of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, 243002, China.
⁴ The Education Ministry Key Lab of Resource Chemistry, Joint International Research Laboratory of Resource Chemistry, Ministry of Education, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Frontiers Science Research Base of Biomimetic Catalysis, and College of Chemistry and Materials Science, Shanghai Frontiers Science Center of Biomimetic Catalysis, Shanghai Normal University, Shanghai, 200234, China. huoyuning@shnu.edu.cn.
⁵ College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China. daixiling2010@shnu.edu.cn.

PMID: 35792981
DOI: 10.1007/s00203-022-03065-8

Abstract

Cu₂O/TiO₂ visible-light photocatalytic composite was successfully synthesized by supercritical solvothermal route. Cu₂O/TiO₂ presented excellent bacterial inactivation activity for Pseudomonas marginalis pv. marginalis, which was related to the concentration of bacteria and the antibacterial time. The highest sterilization ratio reached up to 100% when the bacteria was treated with 80 μg/mL of Cu₂O/TiO₂ photocatalytic composite for 80 min, which could be further proved by the damage of integrity and shrink of the cell membrane in transmission electron microscopy (TEM) image. When the bacterial concentration was 1 × 10⁵ CFU/mL, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined as 16 and 32 μg/mL by agar dilution, respectively. Meanwhile, the production of reactive oxygen species (ROS), glutathione reductase (GR) and glutathione (GSH) of Pseudomonas marginalis pv. marginalis treated by Cu₂O/TiO₂ were determined by DCFH-DA, DTNB and kinetic method, respectively, to evaluate the anti-oxidation capacity of bacteria cell. The enzyme activity of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) in bacteria treated with Cu₂O/TiO₂ were measured to further confirm the overproduction of ROS. Cu₂O/TiO₂ was demonstrated as the excellent visible-light photocatalyst for efficiently killing Pseudomonas marginalis pv. marginalis with the low dosage. Finally, the Cu₂O/TiO₂ composite photocatalytic material was applied to cucumber seedlings based on field experimental, and its inhibitory effect in practical application was judged by measuring the morphology, enzyme activity and resistance index of cucumber plants. It is of great significance to the practical application as a suitable and powerful antibacterial agent for Pseudomonas marginalis pv. marginalis and other bacteria.

Keywords: Cu2O/TiO2 photocatalytic composite; Glutathione reductase; Photocatalytic sterilization; Pseudomonas marginalis pv. marginalis.

MeSH terms

Anti-Bacterial Agents* / pharmacology
Copper* / pharmacology
Pseudomonas
Reactive Oxygen Species
Titanium

Substances

Anti-Bacterial Agents
Reactive Oxygen Species
titanium dioxide
Copper
Titanium

Supplementary concepts

Pseudomonas marginalis

Abstract

MeSH terms

Substances

Supplementary concepts

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