Two Bioactive Compounds, Uniformides A and B, Isolated from a Culture of Nocardia uniformis IFM0856T in the Presence of Animal Cells

Yasumasa Hara; Keiichiro Watanabe; Akiko Takaya; Teruhisa Manome; Takashi Yaguchi; Masami Ishibashi

doi:10.1021/acs.orglett.2c02092

Two Bioactive Compounds, Uniformides A and B, Isolated from a Culture of Nocardia uniformis IFM0856^T in the Presence of Animal Cells

Org Lett. 2022 Jul 15;24(27):4998-5002. doi: 10.1021/acs.orglett.2c02092. Epub 2022 Jul 6.

Authors

Yasumasa Hara^{1

2}, Keiichiro Watanabe¹, Akiko Takaya^{1

2

3}, Teruhisa Manome¹, Takashi Yaguchi³, Masami Ishibashi^{1

2}

Affiliations

¹ Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan.
² Plant Molecular Science Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan.
³ Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.

PMID: 35792521
DOI: 10.1021/acs.orglett.2c02092

Abstract

Two new peptides named uniformides A and B (1 and 2, respectively) were isolated from the cultured extracts of Nocardia uniformis IFM0856^T in the presence of mouse macrophage-like cell line J774.1, in modified Czapek-Dox medium. These compounds were not produced in a culture containing only N. uniformis but in one that also included J774.1. Compounds 1 and 2 showed high cytotoxicity against J774.1 and suppressed the production of nitric oxide, IL-6, and IL-1β by inhibiting the NF-κB pathway.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Lipopolysaccharides / pharmacology
Mice
NF-kappa B / metabolism
Nitric Oxide
Nitric Oxide Synthase Type II / metabolism
Nocardia* / metabolism

Substances

Lipopolysaccharides
NF-kappa B
Nitric Oxide
Nitric Oxide Synthase Type II

Supplementary concepts

Nocardia uniformis