Cultivation of Navicula sp. on rice straw hydrolysate for the production of biogenic silica

Sumit Dhali; Rahul Jain; Anushree Malik; Satyawati Sharma; Ramesh Raliya

doi:10.1016/j.biortech.2022.127577

Cultivation of Navicula sp. on rice straw hydrolysate for the production of biogenic silica

Bioresour Technol. 2022 Sep:360:127577. doi: 10.1016/j.biortech.2022.127577. Epub 2022 Jul 2.

Authors

Sumit Dhali¹, Rahul Jain¹, Anushree Malik², Satyawati Sharma³, Ramesh Raliya⁴

Affiliations

¹ Applied Microbiology Laboratory, Centre for Rural Development and Technology, Indian Institute of Technology Delhi, New Delhi, India.
² Applied Microbiology Laboratory, Centre for Rural Development and Technology, Indian Institute of Technology Delhi, New Delhi, India. Electronic address: anushree@rdat.iitd.ac.in.
³ Biochemistry Laboratory, Centre for Rural Development and Technology, Indian Institute of Technology Delhi, New Delhi, India.
⁴ Adjunct Faculty, Centre for Rural Development and Technology, Indian Institute of Technology Delhi, New Delhi, India.

PMID: 35792331
DOI: 10.1016/j.biortech.2022.127577

Abstract

Rice straw hydrolysate (RSH) prepared at room temperature was found to be rich in silica (140 ± 4.1 mg L^-1) and other nutrients (nitrate-N: 160 ± 4.3 mg L^-1, total dissolve phosphate: 164 ± 6.7 mg L^-1, ammoniacal-N: 439.8 ± 17 mg L^-1). The aim of this work was to study four RSH dilutions (10, 30, 50, 70% v/v) to cultivate Navicula sp. with modified ASN-III as a control. The best result was achieved in 30% RSH in terms ofdoubling time (d = 1.49 days) and growth rate (µ_max = 0.46 day^-1). Compared to control, specific growth rate and biomass productivity were increased by 2.93 folds and 1.85 folds, respectively. Cultivation in 5 L reactor with optimized 30% RSH yielded frustule (54.2 ± 1.9%), carbohydrate (12.4 ± 1.2%), lipid (18.9 ± 1.4%), and protein (8.2 ± 0.6%). The residual solid fraction showed 18.99% increased theoretical methane yield than raw rice straw. Overall, the present process offers a sustainable solution to manage rice straw residue and recover nanoporous silica.

Keywords: Biomass; Bubble column reactor; Frustules; Rice straw hydrolysate; Waste to wealth.

MeSH terms

Biomass
Hydrolysis
Methane / chemistry
Oryza* / chemistry
Silicon Dioxide

Substances

Silicon Dioxide
Methane