Strain-boosted hyperoxic graphene oxide efficiently loading and improving performances of microcystinase

Hong-Lin Liu; Cai Cheng; Ling-Zi Zuo; Ming-Yue Yan; Yan-Lin He; Shi Huang; Ming-Jing Ke; Xiao-Liang Guo; Yu Feng; Hai-Feng Qian; Ling-Ling Feng

doi:10.1016/j.isci.2022.104611

Strain-boosted hyperoxic graphene oxide efficiently loading and improving performances of microcystinase

iScience. 2022 Jun 16;25(7):104611. doi: 10.1016/j.isci.2022.104611. eCollection 2022 Jul 15.

Authors

Affiliations

¹ Key Laboratory of Pesticide & Chemical Biology (CCNU), Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079 Hubei Province, China.
² College of Environment, Zhejiang University of Technology, Hangzhou, 310014 Zhejiang Province, China.

Abstract

Harmful Microcystis blooms (HMBs) and microcystins (MCs) that are produced by Microcystis seriously threaten water ecosystems and human health. This study demonstrates an eco-friendly strategy for simultaneous removal of MCs and HMBs by adopting unique hyperoxic graphene oxides (HGOs) as carrier and pure microcystinase A (PMlrA) as connecting bridge to form stable HGOs@MlrA composite. After oxidation, HGOs yield inherent structural strain effects for boosting the immobilization of MlrA by material characterization and density functional theory calculations. HGO₅ exhibits higher loading capacities for crude MlrA (1,559 mg·g^-1) and pure MlrA (1,659 mg·g^-1). Moreover, the performances of HGO₅@MlrA composite, including the capability of removing MCs and HMBs, the ecological and human safety compared to MlrA or HGO₅ treatment alone, have been studied. These results indicate that HGO₅ can be used as a promising candidate material to effectively improve the application potential of MlrA in the simultaneous removal of MCs and HMBs.

Keywords: Applied microbiology; Materials chemistry; Materials science.