Soil microbiota associated with immune-mediated disease was influenced by heavy metal stress in roadside soils of Shanghai

Abstract

Heavy metals (HMs) and total petroleum hydrocarbons (TPHs) in soils can be detrimental to both soil microorganisms and public health. However, the effects of HMs and TPHs on microbes as well as the consequent microbial-derived health risk remains unclear in soils by local roads where citizens are clearly accessible to traffic-derived pollutants. Herein, we sampled 84 roadside soils throughout Shanghai. We measured the levels of soil edaphic factors, 6 HMs, and alkane TPHs. We further focused on the responses of bacterial and fungal communities assessed via sequencing and network analysis. Results showed that all soil HMs exceeded background levels of Shanghai soil, while the levels of TPHs are comparable to unpolluted sites. Bacterial network nodes and links decreased sharply under HM stress whereas that of fungal networks remained unchanged. The differential pattern was attributed to the asynchronous response of key classes that fungal key classes were more resistant to HMs than bacteria. In addition, 66.8 % of fungal genera associated with immune-mediated disease increased with increased HM stress for its HM tolerance. Together our findings indicate that despite the relatively stable fungal community in response to environmental stresses, the elevation of harmful fungi likely pose threats to health of urban dwellers.

Keywords: Alkane total petroleum hydrocarbon; Heavy metal; Microorganisms associated with immune mediated diseases; Network analysis; Roadside soil.