Loci underlying leaf agronomic traits identified by re-sequencing celery accessions based on an assembled genome

Qing Cheng; Liang Sun; Han Qiao; Zixiong Li; Mingxuan Li; Xiangyun Cui; Wenjie Li; Sujun Liu; Haoran Wang; Wencai Yang; Huolin Shen

doi:10.1016/j.isci.2022.104565

Loci underlying leaf agronomic traits identified by re-sequencing celery accessions based on an assembled genome

iScience. 2022 Jun 9;25(7):104565. doi: 10.1016/j.isci.2022.104565. eCollection 2022 Jul 15.

Authors

Qing Cheng¹, Liang Sun¹, Han Qiao¹, Zixiong Li¹, Mingxuan Li¹, Xiangyun Cui¹, Wenjie Li¹, Sujun Liu¹, Haoran Wang¹, Wencai Yang¹, Huolin Shen¹

Affiliation

¹ Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, China.

Abstract

Celery is one of the most popular vegetables in the world. The main edible parts of celery are the leaf blade and petiole. The celery petiole is usually green, red, or white, with a hollow or solid pith. However, the loci/genes controlling these petiole-related traits have not been reported. In this study, we present a chromosome-level celery genome assembly with a total size of 3.339 Gb. Simultaneous bursts of long-terminal repeats (78.43%) contributed greatly to the large genome size. Re-sequencing and population structure analysis of 79 celery accessions revealed that they could be divided into Chinese celery and Western celery. By combining genome-wide association studies (GWAS) and mapping data, we located the hollow petiole (hp) loci in an 807.6-kb region on chromosome 11. This study provides valuable resources for genetic research on celery and is also helpful for the identification and cloning of genes controlling leaf agronomic traits in celery.

Keywords: Genomics; Omics; Plant Genetics; Plant biology.