The effect of AMP kinase activation on differentiation and maturation of osteoblast cultured on titanium plate

Phanthavong Vansana; Kae Kakura; Yusuke Taniguchi; Kei Egashira; Etsuko Matsuzaki; Takashi Tsutsumi; Hirofumi Kido

doi:10.1016/j.jds.2021.12.003

The effect of AMP kinase activation on differentiation and maturation of osteoblast cultured on titanium plate

J Dent Sci. 2022 Jul;17(3):1225-1231. doi: 10.1016/j.jds.2021.12.003. Epub 2021 Dec 20.

Authors

Phanthavong Vansana¹, Kae Kakura¹, Yusuke Taniguchi¹, Kei Egashira¹, Etsuko Matsuzaki^{2

3}, Takashi Tsutsumi^{3

4}, Hirofumi Kido¹

Affiliations

¹ Section of Oral Implantology, Department of Oral Rehabilitation, Fukuoka Dental College, Fukuoka, Japan.
² Section of Operative Dentistry and Endodontology, Department of Odontology, Fukuoka Dental College, Fukuoka, Japan.
³ Oral Medicine Research Center, Fukuoka Dental College, Fukuoka, Japan.
⁴ The Center for Visiting Dental Service, Department of General Dentistry, Fukuoka Dental College, Fukuoka, Japan.

Abstract

Background/purpose: 5' Adenosine monophosphate-activated protein kinase (AMPK) is known as an enzyme that maintains intracellular homeostasis and has various biological activity. The purpose of this study is evaluation effect of AMPK activation on implant prognosis.

Materials & methods: MC3T3-E1 osteoblast-like cells were cultured on titanium using a 24-well plate. The experimental group was divided into the following 3 groups: (1) the normal culture group (control group), (2) the osteogenic induction group, and (3) the osteogenic induction + AMPK activation group. The cell counts were measured; real-time PCR was used to assess the expression of ALP and Osterix as osteogenic related genes at Day 0,7,14 and 21 after experiments. Additionally, ALP activity and calcification were assessed.

Results: The results of the real-time PCR assessments revealed that the expression of ALP, which is a marker for the initial stages of calcification, was significantly increased by AMPK activation compared to the normal culture or osteogenic induction. A significant increase was also observed in the expression of Osterix, which is a marker for the later stages of calcification. Because significant increases were observed in ALP activity and calcification potential, this suggested that AMPK activation could elicit an increase in osteoblast calcification potential.

Conclusion: AMPK activation promotes implant peripheral osteoblast differentiation and maturation and enhances calcification. Our results suggest that AMPK activation may help to maintain implant stability.

Keywords: 5′ adenosine monophosphate-activated protein kinase; Acadesine/AICA riboside; Dental implant; Osteoblast.