An Antifungal Role of Hydrogen Sulfide on Botryosphaeria Dothidea and Amino Acid Metabolism Involved in Disease Resistance Induced in Postharvest Kiwifruit

Bing Duan; Huaying Du; Wei Zhang; Jing Wang; Zhipeng Cai; Yonggen Shen; Tenghuan Huang; Jie Yuan; Zengyu Gan; Jinyin Chen; Liqin Zhu

doi:10.3389/fpls.2022.888647

An Antifungal Role of Hydrogen Sulfide on Botryosphaeria Dothidea and Amino Acid Metabolism Involved in Disease Resistance Induced in Postharvest Kiwifruit

Front Plant Sci. 2022 Jun 16:13:888647. doi: 10.3389/fpls.2022.888647. eCollection 2022.

Authors

Bing Duan¹, Huaying Du¹, Wei Zhang¹, Jing Wang¹, Zhipeng Cai¹, Yonggen Shen¹, Tenghuan Huang¹, Jie Yuan¹, Zengyu Gan¹, Jinyin Chen^{2

3}, Liqin Zhu^{1

2}

Affiliations

¹ College of Food Science and Technology, Jiangxi Agricultural University, Nanchang, China.
² Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits and Vegetables, Collaborative Innovation Center of Postharvest Key Technology and Quality Safety of Fruits and Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang, China.
³ College of Materials and Chemical Engineering, Pingxiang University, Pingxiang, China.

Abstract

Botryosphaeria dothidea is a major pathogen responsible for postharvest kiwifruit soft rot. This study aimed to determine the influence of hydrogen sulfide (H₂S) on postharvest resistance to kiwifruit soft rot and the antifungal role of H₂S against B. dothidea. The results indicated that H₂S (20 μl L^-1) restricted the lesion area following inoculation with B. dothidea. H₂S enhanced the production of shikimic acid, tyrosine, tryptophan, and phenylalanine while also increasing the total phenols, flavonoids, and lignin. H₂S upregulated the expression of AcDHQS, AcSDH, AcSK, AcPAL, AcCAD, and AcCHS. Additionally, sodium hydrosulfide (NaHS)-released H₂S inhibited mycelial growth. NaHS concentrations of 20 and 40 mmol L^-1 significantly decreased the mycelial weight and malondialdehyde content (MDA) content while increasing cell membrane conductivity and membrane leakage. The results indicate that H₂S induces resistance in kiwifruit via a microbicidal role and amino acid metabolism involved in postharvest kiwifruit disease resistance.

Keywords: Botryosphaeria dothidea; amino acid metabolism; disease resistance; hydrogen sulfide; kiwifruit.