Epidemiology of bla CTX-M-Positive Salmonella Typhimurium From Diarrhoeal Outpatients in Guangdong, China, 2010-2017

Qi Jiang; Bi-Xia Ke; De-Shu Wu; Dong Wang; Liang-Xing Fang; Ruan-Yang Sun; Min-Ge Wang; Jing-Er Lei; Zheng Shao; Xiao-Ping Liao

doi:10.3389/fmicb.2022.865254

Epidemiology of bla _CTX-M-Positive Salmonella Typhimurium From Diarrhoeal Outpatients in Guangdong, China, 2010-2017

Front Microbiol. 2022 Jun 17:13:865254. doi: 10.3389/fmicb.2022.865254. eCollection 2022.

Authors

Qi Jiang^{1

2}, Bi-Xia Ke³, De-Shu Wu^{1

2}, Dong Wang^{1

2}, Liang-Xing Fang^{1

2}, Ruan-Yang Sun^{1

2}, Min-Ge Wang^{1

2}, Jing-Er Lei^{1

2}, Zheng Shao^{1

2}, Xiao-Ping Liao^{1

2

4}

Affiliations

¹ National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China.
² Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
³ Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China.
⁴ Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China.

Abstract

Salmonella enterica can lead to intestinal diarrhea, and the emergence and spread of cephalosporin-resistant Salmonella have brought great challenges to clinical treatment. Therefore, this study investigated the prevalence and transmission of bla _CTX-M genes among S. Typhimurium from diarrhoeal outpatients in Guangdong, China, from 2010 to 2017. A total of 221 bla _CTX-M-positive isolates were recovered from 1,263 S. Typhimurium isolates from the facal samples of diarrhoea patients in 45 general hospitals from 11 cities. The most popular CTX-M gene was bla _CTX-M-55 (39.6%, 72/182) in the CTX-M-1 group, followed by bla _CTX-M-14 (22.5%, 41/182) and bla _CTX-M-65 (19.2%, 35/182) in the CTX-M-9 group. The isolates that carried bla _CTX-M-9G had significantly higher resistance rates to multiple antibacterials compared with bla _CTX-M-1G (p < 0.01). Meanwhile, PFGE analysis not only showed the clonal transmission of bla _{CTX-M-55/14/65}-positve isolates of diarrhoeal outpatients' origins from different hospitals in Guangdong province, but also the characteristic of bla _{CTX-M-55/14/65}-positve isolates' bacterial persistence. Multilocus sequence typing (MLST) analysis indicated that these S. Typhimurium isolates possessed ST34 and ST19. Furthermore, genomic Beast phylogenomic analysis provided the evidence of a close relationship of bla _CTX-M-positive S. Typhimurium isolates between the outpatients and pork. Most bla _{CTX-M-55/14/65} genes were transmitted by non-typeable or IncI1/IncFII/IncHI2 plasmids with the size of ranging from ~80 to ~280 kb. Moreover, whole-genome sequencing (WGS) analysis further revealed that bla _{CTX-M-55/14/65} coexisted with other 25 types of ARGs, of which 11 ARGs were highly prevalent with the detection rates >50%, and it first reported the emergence of bla _TEM-141 in S. Typhimurium. This study underscores the importance of surveillance for bla _CTX-M-positive microbes in diarrhea patients.

Keywords: Guangdong; Salmonella Typhimurium; bacterial persistence; blaCTX-M; diarrhoeal outpatients.