Biofilm Formation and Antimicrobial Susceptibility of E. coli Associated With Colibacillosis Outbreaks in Broiler Chickens From Saskatchewan

Murugesan Sivaranjani; Madeline C McCarthy; Michelle K Sniatynski; Linzhi Wu; Jo-Anne R Dillon; Joseph E Rubin; Aaron P White

doi:10.3389/fmicb.2022.841516

Biofilm Formation and Antimicrobial Susceptibility of E. coli Associated With Colibacillosis Outbreaks in Broiler Chickens From Saskatchewan

Front Microbiol. 2022 Jun 17:13:841516. doi: 10.3389/fmicb.2022.841516. eCollection 2022.

Authors

Murugesan Sivaranjani^{1

2}, Madeline C McCarthy^{1

2}, Michelle K Sniatynski^{1

3}, Linzhi Wu¹, Jo-Anne R Dillon^{1

2}, Joseph E Rubin³, Aaron P White^{1

2}

Affiliations

¹ Vaccine and Infectious Disease Organization, Saskatoon, SK, Canada.
² Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada.
³ Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK, Canada.

Abstract

The global poultry industry has grown to the extent that the number of chickens now well exceeds the number of humans on Earth. Escherichia coli infections in poultry cause significant morbidity and economic losses for producers each year. We obtained 94 E. coli isolates from 12 colibacillosis outbreaks on Saskatchewan farms and screened them for antimicrobial resistance and biofilm formation. Fifty-six isolates were from broilers with confirmed colibacillosis, and 38 isolates were from healthy broilers in the same flocks (cecal E. coli). Resistance to penicillins, tetracyclines, and aminoglycosides was common in isolates from all 12 outbreaks, while cephalosporin resistance varied by outbreak. Most E. coli were able to form biofilms in at least one of three growth media (1/2 TSB, M63, and BHI broth). There was an overall trend that disease-causing E. coli had more antibiotic resistance and were more likely to form biofilms in nutrient-rich media (BHI) as compared to cecal strains. However, on an individual strain basis, there was no correlation between antimicrobial resistance and biofilm formation. The 21 strongest biofilm forming strains consisted of both disease-causing and cecal isolates that were either drug resistant or susceptible. Draft whole genome sequencing indicated that many known antimicrobial resistance genes were present on plasmids, with disease-causing E. coli having more plasmids on average than their cecal counterparts. We tested four common disinfectants for their ability to kill 12 of the best biofilm forming strains. All disinfectants killed single cells effectively, but biofilm cells were more resistant, although the difference was less pronounced for the disinfectants that have multiple modes of action. Our results indicate that there is significant diversity and complexity in E. coli poultry isolates, with different lifestyle pressures affecting disease-causing and cecal isolates.

Keywords: Avian pathogenic E. coli (APEC); antibiotics; antimicrobial resistance (AMR); biofilm; colibacillosis; disinfectant; poultry.