An Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae

Verena Siewers

doi:10.1007/978-1-0716-2399-2_1

An Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae

Methods Mol Biol. 2022:2513:1-13. doi: 10.1007/978-1-0716-2399-2_1.

Author

Verena Siewers¹

Affiliation

¹ Division of Systems and Synthetic Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden. siewers@chalmers.se.

PMID: 35781196
DOI: 10.1007/978-1-0716-2399-2_1

Abstract

For genetic manipulation of yeast, numerous selection marker genes have been employed. These include prototrophic markers, markers conferring drug resistance, autoselection markers, and counterselectable markers. This chapter describes the different classes of selection markers and provides a number of examples for different applications.

Keywords: Autoselection; Auxotrophy; Counterselection; Drug resistance; Marker loop-out.

Publication types

Review

MeSH terms

Biomarkers
Saccharomyces cerevisiae Proteins* / genetics
Saccharomyces cerevisiae* / genetics
Transformation, Genetic

Substances

Biomarkers
Saccharomyces cerevisiae Proteins