Role of extracellular polymeric substances in metal sequestration during mangrove restoration

Abstract

Extracellular polymeric substances (EPS) are widely observed in aquatic ecosystems, however the potential function of EPS on metal sequestration in mangrove wetlands is unclear. Thus, an ecological restoration area (including Sonneratia apetala, Kandelia obovata and unvegetated mudflat) was employed to assess the effect of mangrove reforestation on metal sequestration and the underlying roles played by EPS. The results showed that mangrove restoration directly promoted metal accumulation (e.g., Cr, Cu, Ni, Pb, and Zn) in sediments. However, alleviated metal bioavailability was detected after mangrove reforestation. The changes in metal accumulation and bioavailability were highly correlated with EPS and microbial composition. Mangrove restoration (especially for K. obovata reforestation) also significantly promoted EPS production, in which multiple metal-chelating functional groups (e.g., hydroxyl, carboxyl, and imino) were identified by Fourier infrared spectra. Moreover, the contents of EPS were positively correlated with metal accumulation but negatively correlated with metal bioavailability. The present data further illustrated that the enhancements of Gammaproteobacteria, Bacteroidia, Desulfobulbia, and Desulfobacteria might be important for EPS production. In summary, this is the first study to reveal that the presence of artificial mangroves might act as an efficient barrier in metal sequestration and immobilization by enhancing inherent microbial EPS.

Keywords: Extracellular polymeric substances; Heavy metals; Kandelia obovata.; Mangrove; Sonneratia apetala.