3D tracking of ferrous iron in the epileptic mouse brain and screening of iron homeostasis regulators with a two-photon fluorescent probe

Chenwen Shao; Zhang-Peng Chen; Chao Yan; Yong Qian

doi:10.1016/j.xpro.2022.101522

3D tracking of ferrous iron in the epileptic mouse brain and screening of iron homeostasis regulators with a two-photon fluorescent probe

STAR Protoc. 2022 Sep 16;3(3):101522. doi: 10.1016/j.xpro.2022.101522. Epub 2022 Jul 1.

Authors

Chenwen Shao¹, Zhang-Peng Chen², Chao Yan³, Yong Qian⁴

Affiliations

¹ Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Wenyuan Road 1, Nanjing 210023, China; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Xianlin Road 163, Nanjing 210023, China; Wuxi School of Medicine and School of Food Science and Technology, Jiangnan University, Jiangsu, China.
² State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Xianlin Road 163, Nanjing 210023, China.
³ State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Xianlin Road 163, Nanjing 210023, China. Electronic address: yanchao@nju.edu.cn.
⁴ Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Wenyuan Road 1, Nanjing 210023, China. Electronic address: yongqian@njnu.edu.cn.

Abstract

The development of techniques for tracking active ferrous iron (Fe²⁺) distribution has greatly promoted the biological studies of iron. Here, we present an innovative application of a 3D two-photon fluorescent probe for Fe²⁺ tracking in the epileptic mouse brain, which has expanded the toolbox of screening for iron homeostasis regulators and contributed to the discovery of new chemical entities for the treatment of epilepsy. For complete details on the use and execution of this protocol, please refer to Shao et al. (2022).

Keywords: Chemistry; Health Sciences.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / diagnostic imaging
Epilepsy* / drug therapy
Ferrous Compounds
Fluorescent Dyes
Homeostasis
Iron*
Mice

Substances

Ferrous Compounds
Fluorescent Dyes
Iron