Electrochemical DNA biosensor for chronic myelocytic leukemia based on hybrid nanostructure

Karen Y P S Avelino; Léony S Oliveira; Maryana R Santos; Norma Lucena-Silva; César A S Andrade; Maria D L Oliveira

doi:10.1016/j.bioelechem.2022.108176

Electrochemical DNA biosensor for chronic myelocytic leukemia based on hybrid nanostructure

Bioelectrochemistry. 2022 Oct:147:108176. doi: 10.1016/j.bioelechem.2022.108176. Epub 2022 Jun 8.

Authors

Karen Y P S Avelino¹, Léony S Oliveira¹, Maryana R Santos², Norma Lucena-Silva³, César A S Andrade¹, Maria D L Oliveira⁴

Affiliations

¹ Programa de Pós-Graduação em Inovação Terapêutica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil; Laboratório de Biodispositivos Nanoestruturados, Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
² Laboratório de Biodispositivos Nanoestruturados, Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
³ Instituto Aggeu Magalhães, Fundação Oswaldo Cruz (Fiocruz), 50670-420 Recife, PE, Brazil; Laboratório de Biologia Molecular, Departamento de Oncologia Pediátrica, Instituto de Medicina Integral Professor Fernando Figueira (IMIP), 50070-550 Recife, PE, Brazil.
⁴ Programa de Pós-Graduação em Inovação Terapêutica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil; Laboratório de Biodispositivos Nanoestruturados, Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil. Electronic address: maria.dloliveira@ufpe.br.

PMID: 35779517
DOI: 10.1016/j.bioelechem.2022.108176

Abstract

The present research refers to elaborating a new label-free electrochemical biosensor used to detect the BCR/ABL fusion gene. We used a hybrid nanocomposite composed of chitosan and zinc oxide nanoparticles (Chit-ZnONP) immobilized on a polypyrrole (PPy) film. DNA segments were covalently immobilized, allowing biomolecular recognition. Atomic force microscopy (AFM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) were used to evaluate the assembly stages of the biosensor. The biosensor's analytical performance was investigated using recombinant plasmids containing the target oncogene and clinical samples from patients with chronic myeloid leukemia (CML). A limit of detection (LOD) of 1.34 fM, limit of quantification (LOQ) of 4.08 fM, and sensitivity of 34.03 μA fM^-1 cm² were calculated for the BCR/ABL fusion oncogene. The sensing system exhibited high specificity, selectivity, and reproducibility with a standard deviation (SD) of 4.21%. Additionally, a linear response range was observed between 138.80 aM to 13.88 pM with a regression coefficient of 0.96. Also, the biosensor shows easy operationalization and fast analytical response, contributing to the early cancer diagnosis. The proposed nanostructured device is an alternative for the genetic identification BCR/ABL fusion gene.

Keywords: BCR/ABL fusion gene; Biosensor; Electrochemistry; Hybrid nanocomposite; Polypyrrole; Zinc oxide.

MeSH terms

Biosensing Techniques* / methods
DNA / genetics
Electrochemical Techniques / methods
Humans
Leukemia, Myelogenous, Chronic, BCR-ABL Positive* / diagnosis
Leukemia, Myelogenous, Chronic, BCR-ABL Positive* / genetics
Nanocomposites* / chemistry
Polymers / chemistry
Pyrroles
Reproducibility of Results

Substances

Polymers
Pyrroles
DNA