Protocol of living cell separation using the microfluidic dielectrophoresis integrated chip

Kazumi Koba; Kyoko Yarimizu; So Fujiyoshi; Kyoichi Oshiro; Yoshikazu Wakizaka; Masayo Takano; Fumito Maruyama

doi:10.1016/j.xpro.2022.101527

Protocol of living cell separation using the microfluidic dielectrophoresis integrated chip

STAR Protoc. 2022 Sep 16;3(3):101527. doi: 10.1016/j.xpro.2022.101527. Epub 2022 Jul 1.

Authors

Kazumi Koba¹, Kyoko Yarimizu¹, So Fujiyoshi¹, Kyoichi Oshiro², Yoshikazu Wakizaka², Masayo Takano², Fumito Maruyama³

Affiliations

¹ Microbial Genomics and Ecology, The IDEC Institute, Hiroshima University, 1-3-2 Kagamiyama, Higashi-Hiroshima City, Hiroshima 739-8511, Japan.
² AFI Corporation, Medical Innovation Center Bldg. 2nd Floor of Kyoto University, 53 Shogoin Kawahara-cho, Sakyo-ku, Kyoto City 606-8507, Japan.
³ Microbial Genomics and Ecology, The IDEC Institute, Hiroshima University, 1-3-2 Kagamiyama, Higashi-Hiroshima City, Hiroshima 739-8511, Japan. Electronic address: fumito@hiroshima-u.ac.jp.

Abstract

This protocol demonstrates the separation of living cells with the microfluidic dielectrophoresis chip, using the Jurkat cell as a model. The successful living cell separation lies in familiarity with the detailed tips, which are aided by this stepwise protocol. The knowledge of correct chip installation, sample and buffer filling, flow rate and cell concentration adjustments, and contamination sources increases the efficiency of target viable cell collection. Such instructions, although trivial, are critical for achieving cell separation. For complete details on the use and execution of this protocol, please refer to Oshiro et al. (2022).

Keywords: Biotechnology and bioengineering; Cell Biology; Cell isolation; Cell separation/fractionation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Separation / methods
Microfluidic Analytical Techniques* / methods
Microfluidics