Application of potential probiotic strain Streptomyces sp. SH5 on anti-Aeromonas infection in zebrafish larvae

Qiting Liang; Ganxing Liu; Zheng Guo; Yuting Wang; Zhongheng Xu; Yuxian Ren; Qizhong Zhang; Miao Cui; Xinqing Zhao; Delin Xu

doi:10.1016/j.fsi.2022.06.049

Application of potential probiotic strain Streptomyces sp. SH5 on anti-Aeromonas infection in zebrafish larvae

Fish Shellfish Immunol. 2022 Aug:127:375-385. doi: 10.1016/j.fsi.2022.06.049. Epub 2022 Jun 28.

Authors

Qiting Liang¹, Ganxing Liu², Zheng Guo², Yuting Wang², Zhongheng Xu², Yuxian Ren², Qizhong Zhang³, Miao Cui⁴, Xinqing Zhao⁵, Delin Xu⁶

Affiliations

¹ Department of Ecology, Institute of Hydrobiology, School of Life Science and Technology, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Jinan University, Guangzhou, 510632, PR China; State Environmental Protection Key Laboratory of Drinking Water Source Management and Technology, Shenzhen Key Laboratory of Drinking Water Source Safety Control, Shenzhen Academy of Environmental Sciences, Shenzhen, 518001, PR China.
² Department of Ecology, Institute of Hydrobiology, School of Life Science and Technology, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Jinan University, Guangzhou, 510632, PR China.
³ Department of Ecology, Institute of Hydrobiology, School of Life Science and Technology, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Jinan University, Guangzhou, 510632, PR China. Electronic address: zhangqzdr@126.com.
⁴ Department of Ecology, Institute of Hydrobiology, School of Life Science and Technology, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Jinan University, Guangzhou, 510632, PR China. Electronic address: cuisanshui@163.com.
⁵ State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, PR China. Electronic address: xqzhao@sjtu.edu.cn.
⁶ Department of Ecology, Institute of Hydrobiology, School of Life Science and Technology, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Jinan University, Guangzhou, 510632, PR China. Electronic address: xudelin@live.com.

PMID: 35777708
DOI: 10.1016/j.fsi.2022.06.049

Abstract

Pre-treatment of Streptomyces sp. SH5 on zebrafish lead to a significant enhancement of larvae survival upon Aeromonas hydrophila challenging. SH5 was able to colonize in zebrafish approximately at 1 × 10^2.6 cells per fish for at least seven days. The presence of SH5 strongly repelled the A. hydrophila colonization in zebrafish, and maximally, a 67.53% reduction rate was achieved. A more diversified flora was discovered in the SH5-treated zebrafish larvae at both phylum and genus levels. The expression of immune response genes of SH5-treated zebrafish, including TLR3, lysozyme and NOS2α, were enhanced at initial stage, while, that of various inflammatory stimuli genes including 1L-1β, 1L-6 and MyD88 were decreased at all tested timepoints. SH5 was shown to inhibit virulence factors production and the expression of corresponding virulence genes in A. hydrophila, suggesting its quorum sensing inhibitory potential. These results indicated favorable application perspectives of SH5 in resisting pathogenic infection in aquaculture.

Keywords: Aeromonas hydrophila; Aquaculture; Probiotics; Streptomyces; Zebrafish.

MeSH terms

Aeromonas hydrophila / physiology
Aeromonas*
Animals
Fish Diseases*
Gram-Negative Bacterial Infections*
Larva
Probiotics* / pharmacology
Streptomyces* / genetics
Zebrafish