Protocol for detecting substrates in living cells by targeted molecular probes through hyperpolarized 129Xe MRI

Qingbin Zeng; Qianni Guo; Yaping Yuan; Lei Zhang; Weiping Jiang; Yuqi Yang; Xin Zhou

doi:10.1016/j.xpro.2022.101499

Protocol for detecting substrates in living cells by targeted molecular probes through hyperpolarized ¹²⁹Xe MRI

STAR Protoc. 2022 Sep 16;3(3):101499. doi: 10.1016/j.xpro.2022.101499. Epub 2022 Jun 23.

Authors

Qingbin Zeng¹, Qianni Guo², Yaping Yuan³, Lei Zhang², Weiping Jiang¹, Yuqi Yang¹, Xin Zhou⁴

Affiliations

¹ Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences-Wuhan National Laboratory for Optoelectronics, Wuhan 430071, P. R. China; University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
² Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences-Wuhan National Laboratory for Optoelectronics, Wuhan 430071, P. R. China; University of Chinese Academy of Sciences, Beijing 100049, P. R. China; Optics Valley Laboratory, Hubei 430074, P.R. China.
³ Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences-Wuhan National Laboratory for Optoelectronics, Wuhan 430071, P. R. China.
⁴ Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences-Wuhan National Laboratory for Optoelectronics, Wuhan 430071, P. R. China; University of Chinese Academy of Sciences, Beijing 100049, P. R. China; Optics Valley Laboratory, Hubei 430074, P.R. China; Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Haikou 570228, P. R. China. Electronic address: xinzhou@wipm.ac.cn.

Abstract

Due to limited detection sensitivity and contrast limitation, imaging substrates with ¹²⁹Xe MRI in living cells is still a challenge. Here, we present an effective protocol to detect and image substrates in human lung cancer cells A549 with hyperpolarized ¹²⁹Xe MRI. This protocol was optimized for a cryptophane-based probe sensitive to biothiols and can be expanded to other Xe-based probes to detect potential biomarkers in other mammalian cells. For complete details on the use and execution of this protocol, please refer to Zeng et al. (2021).

Keywords: Cell Biology; Chemistry; Molecular/Chemical Probes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Humans
Lung Neoplasms* / diagnostic imaging
Magnetic Resonance Imaging / methods
Mammals
Molecular Probes
Xenon Isotopes* / chemistry

Substances

Molecular Probes
Xenon Isotopes