Characterization of P2X7 Receptors in Human Blood Cells

Laura Hurtado-Navarro; Alberto Baroja-Mazo; Pablo Pelegrín

doi:10.1007/978-1-0716-2384-8_15

Characterization of P2X7 Receptors in Human Blood Cells

Methods Mol Biol. 2022:2510:279-290. doi: 10.1007/978-1-0716-2384-8_15.

Authors

Laura Hurtado-Navarro¹, Alberto Baroja-Mazo¹, Pablo Pelegrín²

Affiliations

¹ Biomedical Research Institute of Murcia (IMIB), Clinical University Hospital Virgen de la Arrixaca, Murcia, Spain.
² Department of Biochemistry and Molecular Biology B and Immunology, University of Murcia, Murcia, Spain. pablo.pelegrin@imib.es.

PMID: 35776331
DOI: 10.1007/978-1-0716-2384-8_15

Abstract

P2X7 receptors are expressed in different circulating immune cells. Depending on the cell in which they are expressed, their function could vary from activation of the leucine-rich repeat receptor pyrin domain containing 3 (NLRP3) inflammasome or the shedding of different surface receptors. The P2X7 receptor has been involved in the evolution of several inflammatory diseases, and thus its characterization in blood samples is important to investigate its role in human diseases. In this chapter, we present different methods to detect the presence and the activation of P2X7 receptors in whole blood samples. These methods could be used to evaluate the degree of P2X7 receptor activation in blood samples from patients suffering different chronic inflammatory diseases.

Keywords: ASC; ATP; CD62-L; Flow cytometry; Inflammasome; L-selectin; Monocytes; NK cells; NLRP3.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blood Cells / metabolism
Carrier Proteins / metabolism
Humans
Inflammasomes / metabolism
NLR Family, Pyrin Domain-Containing 3 Protein* / metabolism
Receptors, Purinergic P2X7* / genetics

Substances

Carrier Proteins
Inflammasomes
NLR Family, Pyrin Domain-Containing 3 Protein
Receptors, Purinergic P2X7