An ICT-Based Coumarin Fluorescent Probe for the Detection of Hydrazine and Its Application in Environmental Water Samples and Organisms

Xina Liu; Meiqing Zhu; Chenyang Xu; Fugang Fan; Panpan Chen; Yi Wang; Dongyang Li

doi:10.3389/fbioe.2022.937489

An ICT-Based Coumarin Fluorescent Probe for the Detection of Hydrazine and Its Application in Environmental Water Samples and Organisms

Front Bioeng Biotechnol. 2022 Jun 14:10:937489. doi: 10.3389/fbioe.2022.937489. eCollection 2022.

Authors

Xina Liu¹, Meiqing Zhu², Chenyang Xu¹, Fugang Fan¹, Panpan Chen¹, Yi Wang¹, Dongyang Li³

Affiliations

¹ Anhui Provincial Key Laboratory of Quality and Safety of Agricultural Products, College of Resources and Environment, Anhui Agricultural University, Hefei, China.
² School of Chemical and Environmental Engineering, Anhui Polytechnic University, Wuhu, China.
³ Laboratory of Agricultural Information Intelligent Sensing, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.

Abstract

As an inorganic small molecule pollutant, the toxicity and potential carcinogenicity of hydrazine (N₂H₄) are of increasing concern. In this work, A water-soluble fluorescent probe (OCYB) based on the intramolecular charge transfer (ICT) mechanism for the detection of hydrazine was designed and synthesized. Taking the advantage of 4-bromobutyryl as the recognition group, the high selectivity of OCYB to N₂H₄ was confirmed by steady-state fluorescence spectroscopy. The limit of detection (LOD) was calculated to be 78 nM in the DMSO-HEPES (pH 7.4) system. The detection mechanism was verified by NMR, HRMS and density functional theory (DFT) calculations. In addition, OCYB exhibits strong anti-interference ability and an "Off-On" fluorescence enhancement effect. Importantly, OCYB can be used to effectively monitor the fluorescence distribution of N₂H₄ in environmental water samples and organisms.

Keywords: density functional theory; detection; fluorescent probe; hydrazine; imaging.