Mismatches in 16S rRNA Gene Primers: An Area Worth Further Exploring

Front Microbiol. 2022 Jun 13:13:888803. doi: 10.3389/fmicb.2022.888803. eCollection 2022.

Wenting Ren¹, Yingwen Zhong^{1

2}, Yi Ding^{1

2}, Yuehong Wu^{1

2}, XueWei Xu^{1

2}, Peng Zhou¹

¹ Key Laboratory of Marine Ecosystem Dynamics, Ministry of Natural Resources and Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, China.
² School of Oceanography, Shanghai Jiao Tong University, Shanghai, China.

No abstract available

Keywords: 16S rRNA gene amplicon sequencing; metagenomic sequencing; microbiome; mismatch; universal primers.