Imaging neuronal migration and network activity in human forebrain assembloids

Fikri Birey; Sergiu P Pașca

doi:10.1016/j.xpro.2022.101478

Imaging neuronal migration and network activity in human forebrain assembloids

STAR Protoc. 2022 Jun 17;3(3):101478. doi: 10.1016/j.xpro.2022.101478. eCollection 2022 Sep 16.

Authors

Fikri Birey¹, Sergiu P Pașca²

Affiliations

¹ Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA; Stanford Brain Organogenesis, Wu Tsai Neurosciences Institute and Bio-X, Stanford University, Stanford, CA, USA.
² Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA; Stanford Brain Organogenesis, Wu Tsai Neurosciences Institute and Bio-X, Stanford University, Stanford, CA, USA. Electronic address: spasca@stanford.edu.

Abstract

Assembloids generated from human pluripotent stem cells are self-organizing, multicellular in vitro models that recapitulate aspects of cell-cell interactions and circuit assembly during neural development. Here, we present protocols to functionally monitor, in forebrain assembloids, the migration of GABAergic interneurons from the ventral to the dorsal forebrain and the activity in early cortical networks. Specifically, we describe high-resolution imaging and analysis of neuronal migration as well as calcium imaging of network activity in forebrain assembloids. For complete details on the use and execution of this protocol, please refer to Birey et al. (2022).

Keywords: Cell Biology; Neuroscience; Organoids.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Cell Movement
Humans
Neurogenesis
Organoids*
Pluripotent Stem Cells*
Prosencephalon

Abstract

Publication types

MeSH terms

Grants and funding