Simultaneous detection of lysosomal SO2 and viscosity using a hemicyanine-based fluorescent probe

Xiao-Hong Li; Xue-Feng Han; Wei-Na Wu; Xiao-Lei Zhao; Yuan Wang; Yun-Chang Fan; Zhi-Hong Xu

doi:10.1016/j.saa.2022.121519

Simultaneous detection of lysosomal SO₂ and viscosity using a hemicyanine-based fluorescent probe

Spectrochim Acta A Mol Biomol Spectrosc. 2022 Nov 5:280:121519. doi: 10.1016/j.saa.2022.121519. Epub 2022 Jun 17.

Authors

Xiao-Hong Li¹, Xue-Feng Han², Wei-Na Wu³, Xiao-Lei Zhao¹, Yuan Wang⁴, Yun-Chang Fan¹, Zhi-Hong Xu⁵

Affiliations

¹ College of Chemistry and Chemical Engineering, Henan Key Laboratory of Coal Green Conversion, Henan Polytechnic University, Jiaozuo 454000, PR China.
² College of Safety Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, PR China.
³ College of Chemistry and Chemical Engineering, Henan Key Laboratory of Coal Green Conversion, Henan Polytechnic University, Jiaozuo 454000, PR China. Electronic address: wuwn08@hpu.edu.cn.
⁴ College of Chemistry and Chemical Engineering, Henan Key Laboratory of Coal Green Conversion, Henan Polytechnic University, Jiaozuo 454000, PR China. Electronic address: wangyuan08@hpu.edu.cn.
⁵ Key Laboratory of Chemo/Biosensing and Detection, College of Chemical and Materials Engineering, Xuchang University, Xuchang 461000, PR China; College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450052, PR China. Electronic address: xuzhihong1980@xcu.edu.cn.

PMID: 35763947
DOI: 10.1016/j.saa.2022.121519

Abstract

The changes in sulfur dioxide and viscosity of lysosomes are significant indicators in physiological processes and the cell microenvironment. This study aimed to synthesize a hemicyanine-based probe for simultaneous detection of SO₂ and viscosity. The probe could not only rationally detect sulfur dioxide in a semi-aqueous solution with high sensitivity (limit of detection = 0.78 μM) and fast response (within 30 s) but also monitor viscosity via fluorescence emission enhancement at 580 nm. Further, the dual-response probe was successfully used to image SO₂ and viscosity in the lysosomes of living cells.

Keywords: Dual-response probe; Hemicyanine; Lysosomes; Sulfur dioxide; Viscosity.

MeSH terms

Carbocyanines
Fluorescent Dyes*
HeLa Cells
Humans
Lysosomes
Sulfur Dioxide*
Viscosity

Substances

Carbocyanines
Fluorescent Dyes
tetramethylene hemicyanine
Sulfur Dioxide