Chemical profiling of marine seaweed Halimeda gracilis using UPLC-ESI-Q-TOF-MSE and evaluation of anticancer activity targeting PI3K/AKT and intrinsic apoptosis signaling pathway

Vaikundamoorthy Ramalingam; Nagendla Narendra Kumar; Mohan Harshavardhan; Halmuthur Mahabalarao Sampath Kumar; Ashok Kumar Tiwari; Kattragadda Suresh Babu; Mohana Krishna Reddy Mudiam

doi:10.1016/j.foodres.2022.111394

Chemical profiling of marine seaweed Halimeda gracilis using UPLC-ESI-Q-TOF-MS^E and evaluation of anticancer activity targeting PI3K/AKT and intrinsic apoptosis signaling pathway

Food Res Int. 2022 Jul:157:111394. doi: 10.1016/j.foodres.2022.111394. Epub 2022 May 23.

Authors

Vaikundamoorthy Ramalingam¹, Nagendla Narendra Kumar², Mohan Harshavardhan³, Halmuthur Mahabalarao Sampath Kumar⁴, Ashok Kumar Tiwari⁵, Kattragadda Suresh Babu⁵, Mohana Krishna Reddy Mudiam²

Affiliations

¹ Centre for Natural Products & Traditional Knowledge, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India. Electronic address: ramalingam@iict.res.in.
² Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India; Analytical Chemistry, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India.
³ Department of Chemistry, Jeonbuk National University, Jeonju, South Korea.
⁴ Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India; Organic Synthesis and Process Chemistry, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India.
⁵ Centre for Natural Products & Traditional Knowledge, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.

PMID: 35761649
DOI: 10.1016/j.foodres.2022.111394

Abstract

Marine seaweeds are predominant for nutraceuticals and have been food source since ancient times and are currently being used in Chinese medicines and Japanese traditional medicines. In the present study, the chemical profile analysis of Halimeda gracilis was performed using UPLC-ESI-Q-TOF-MS^E analysis and assessed for its anticancer activity. A cursory investigation of the total ion chromatograms of the both methanol (MHG) and ethyl acetate (EAHG) of H. gracilis extracts reveals that both extracts have different kind of metabolites including phenols and its derivatives, diterpenes, cinnamic acids derivatives etc. The in vitro anticancer activity of EAHG and MHG exhibiting significant activity and induced apoptosis against skin cancer cells by generating excessive ROS, damaging the mitochondrial membrane and nuclear components. Further, the western blot analysis showed that the EAHG and MHG downregulates the oncoproteins PI3K, AKT, p-AKT, and BcL2 and upregulates the apoptotic proteins Bax, Cyto C, p21, p53, Caspase 9 and Caspase 3. To best of our knowledge, this is the first report which implies the UPLC-ESI-Q-TOF-MS^E based chemical profiling of H. gracilis and can be used for the treatment of cancer.

Keywords: Anticancer activity; Apoptosis signaling pathway; Chemical profiling; Halimeda gracilis; Skin cancer; UPLC-ESI-Q-TOF-MS(E).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis
Chromatography, High Pressure Liquid
Drugs, Chinese Herbal*
Phosphatidylinositol 3-Kinases
Proto-Oncogene Proteins c-akt
Seaweed* / chemistry
Signal Transduction

Substances

Drugs, Chinese Herbal
Proto-Oncogene Proteins c-akt