Adolescent use of potential novel probiotic Rouxiella badensis subsp. acadiensis (Canan SV-53) mitigates pubertal LPS-Induced behavioral changes in adulthood in a sex-specific manner by modulating 5HT1A receptors expression in specific brain areas

Nour Yahfoufi; Emily G Ah-Yen; Rajini Chandrasegaram; Sarah Aly; Michael Murack; Anthony K Kadamani; Chantal Matar; Nafissa Ismail

doi:10.1016/j.cpnec.2021.100063

Adolescent use of potential novel probiotic Rouxiella badensis subsp. acadiensis (Canan SV-53) mitigates pubertal LPS-Induced behavioral changes in adulthood in a sex-specific manner by modulating 5HT1A receptors expression in specific brain areas

Compr Psychoneuroendocrinol. 2021 Jun 5:7:100063. doi: 10.1016/j.cpnec.2021.100063. eCollection 2021 Aug.

Authors

Nour Yahfoufi^{1

2}, Emily G Ah-Yen², Rajini Chandrasegaram³, Sarah Aly², Michael Murack², Anthony K Kadamani², Chantal Matar^{1

4}, Nafissa Ismail^{2

5}

Affiliations

¹ Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ontario, Canada.
² NISE Laboratory, School of Psychology, Faculty of Social Sciences, University of Ottawa, Ontario, Canada.
³ Department of Neuroscience, Faculty of Health Sciences, University of Cardiff, Cardiff, UK.
⁴ School of Nutrition, Faculty of Health Sciences, University of Ottawa, Ontario, Canada.
⁵ Brain and Mind Research Institute, University of Ottawa, Ottawa, Ontario, Canada.

Abstract

Adolescence is a critical period of development during which the brain undergoes significant remodeling that impacts behavior later in life. Exposure to stress, and especially immune challenge, during this period triggers changes in brain function resulting in the development of mental disorders in adulthood, such as depression and anxiety. Previous studies from our laboratory have shown that a single exposure to LPS (lipopolysaccharide) during puberty causes enduring depression-like behaviour in females and anxiety-like behaviours in males. However, administration of probiotics during puberty blocked the enduring effects of LPS on depression-like and anxiety-like behaviors in female and male mice, respectively. These results suggest that the gut microbiome is a mediator of the effects of stress on mental health. The objective of the current study is to examine the effectiveness of a novel probiotic Rouxiella badensis subsp. acadiensis (Canan SV-53) in blocking LPS-induced anxiety-like and depression-like behaviors in adult male and female mice. Our results showed that Rouxiella badensis subsp. acadiensis (Canan SV-53) blocked LPS-induced depression-like behavior in female mice. We also found that pubertal treatment with Rouxiella badensis subsp. acadiensis (Canan SV-53) mitigated the LPS-induced decrease in 5HT1A expression in CA1 as well as the LPS-induced increase in 5HT1A expression in the raphe-nuclei in female mice. Contrary to our predictions, pubertal LPS treatment at 6 weeks of age did not induce enduring anxiety-like behavior in males. There was also no difference in anxiety-like behavior between the LPS-sucrose and LPS-probiotic male groups. However, pubertal LPS treatment increased the expression of 5HT1A receptors in the DRN in males, while probiotic exposure mitigated this increase. Our study highlights the consequences of stress exposure (immune challenge) on mental health in adulthood taking into consideration 5HT1A receptors expression at different regions of the brain. It also emphasizes on the importance of considering adolescence as window of opportunities during which probiotic use can alleviate the long-term neural and behavioral alterations induced by stress.

Keywords: 5HT1A; Anxiety; Depression; Hippocampus; Probiotic; Raphé-nuclei.