Inflammatory response of gut, spleen, and liver in mice induced by orally administered Porphyromonas gingivalis

Yingman Liu; Wenkai Huang; Ke Dai; Ni Liu; Jiaqi Wang; Xiaoying Lu; Jiaojiao Ma; Manman Zhang; Mengqi Xu; Xu Long; Jie Liu; Yurong Kou

doi:10.1080/20002297.2022.2088936

Inflammatory response of gut, spleen, and liver in mice induced by orally administered Porphyromonas gingivalis

J Oral Microbiol. 2022 Jun 16;14(1):2088936. doi: 10.1080/20002297.2022.2088936. eCollection 2022.

Authors

Yingman Liu¹, Wenkai Huang², Ke Dai³, Ni Liu¹, Jiaqi Wang¹, Xiaoying Lu⁴, Jiaojiao Ma¹, Manman Zhang⁴, Mengqi Xu¹, Xu Long⁴, Jie Liu⁵, Yurong Kou^{1

4}

Affiliations

¹ Department of Periodontics, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, Liaoning, China.
² Department of Orthodontics, School of Stomatology, China Medical University, Shenyang, Liaoning, China.
³ Department of Stomatology, Lishui University School of Medicine, Lishui, Zhejing, China.
⁴ Department of Oral Biology, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, Liaoning, China.
⁵ Department of Stomatology, Science Experiment Center, China Medical University, Shenyang, Liaoning, China.

Abstract

Background: Periodontitis is a chronic multifactorial inflammatory disease. Porphyromonas gingivalis is a primary periopathogen in the initiation and development of periodontal disease. Evidence has shown that P. gingivalis is associated with systemic diseases, including IBD and fatty liver disease. Inflammatory response is a key feature of diseases related to this species.

Methods: C57BL/6 mice were administered either PBS, or P. gingivalis. After 9 weeks, the inflammatory response in gut, spleen, and liver was analyzed.

Results: The findings revealed significant disturbance of the intestinal microbiota and increased inflammatory factors in the gut of P. gingivalis-administered mice. Administrated P. gingivalis remarkably promoted the secretion of IRF-1 and activated the inflammatory pathway IFN-γ/STAT1 in the spleen. Histologically, mice treated with P. gingivalis exhibited hepatocyte damage and lipid deposition. The inflammatory factors IL-17a, IL-6, and ROR-γt were also upregulated in the liver of mice fed with P. gingivalis. Lee's index, spleen index, and liver index were also increased.

Conclusion: These results suggest that administrated P. gingivalis evokes inflammation in gut, spleen, and liver, which might promote the progression of various systemic diseases.

Keywords: Porphyromonas gingivalis; immune response; inflammation; intestinal bacteria; periodontal disease.

Grants and funding

This work was supported by the Natural Science Foundation of Liaoning province [20180551232]; Science and Technology Project of Shenyang [F16-102-4-00].