A protocol for the generation of EPO - Producing neural crest cells from human induced pluripotent stem cells

Angelo Michele Lavecchia; Sara Buttò; Christodoulos Xinaris

doi:10.1016/j.mex.2022.101753

A protocol for the generation of EPO - Producing neural crest cells from human induced pluripotent stem cells

MethodsX. 2022 Jun 9:9:101753. doi: 10.1016/j.mex.2022.101753. eCollection 2022.

Authors

Angelo Michele Lavecchia¹, Sara Buttò¹, Christodoulos Xinaris¹

Affiliation

¹ Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Bergamo, Italy.

Abstract

Insufficient production of erythropoietin (EPO) leads to anaemia. Developing methods for the generation and transplantation of EPO-producing cells would allow scientists to design personalised therapeutic solutions. Here we present a simple and highly reproducible protocol for the generation of neural crest cells (NCCs) that can produce and secrete erythropoiesis-competent EPO in response to hypoxia.

Keywords: Anaemia; Erythropoietin; Human induced pluripotent stem cells; Neural crest cells.