Nematicidal Activity of Burkholderia arboris J211 Against Meloidogyne incognita on Tobacco

Renjun Zhang; Jin Ouyang; Xingyang Xu; Jie Li; Muzammal Rehman; Gang Deng; Jie Shu; Dake Zhao; Suiyun Chen; R Z Sayyed; Shah Fahad; Yaqiong Chen

doi:10.3389/fmicb.2022.915546

Nematicidal Activity of Burkholderia arboris J211 Against Meloidogyne incognita on Tobacco

Front Microbiol. 2022 Jun 10:13:915546. doi: 10.3389/fmicb.2022.915546. eCollection 2022.

Authors

Renjun Zhang^{1

2

3}, Jin Ouyang⁴, Xingyang Xu⁴, Jie Li⁴, Muzammal Rehman⁵, Gang Deng⁵, Jie Shu³, Dake Zhao^{1

2

6}, Suiyun Chen^{1

2

6}, R Z Sayyed⁷, Shah Fahad^{8

9}, Yaqiong Chen⁴

Affiliations

¹ Biocontrol Engineering Research Center of Crop Disease and Pest, Yunnan University, Kunming, China.
² Yunnan Engineering Research Center of Microbial Agents, Yunnan University, Kunming, China.
³ School of Life Science, Yunnan University, Kunming, China.
⁴ Kunming Branch of Yunnan Tobacco Company, Kunming, China.
⁵ School of Agriculture, Yunnan University, Kunming, China.
⁶ School of Ecology and Environmental Science, Yunnan University, Kunming, China.
⁷ Department of Microbiology, PSGVP Mandal's S I Patil Arts, G B Patel Science and STKVS Commerce College, Shahada, India.
⁸ Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Tropical Crops, Hainan University, Haikou, China.
⁹ Department of Agronomy, The University of Haripur, Haripur, Pakistan.

Abstract

Root-knot nematode (Meloidogyne incognita) is the most widespread nematode affecting Solanaceae crops. Due to the lack of effective measures to control this nematode, its management can be achieved, using biocontrol agents. This study investigated in vitro efficacy of the antagonistic bacterial strain J211 isolated from tobacco rhizosphere soil against M. incognita, and further assessed its role in controlling nematodes, both in pot and field trials. Phylogenetic analysis of the 16S rRNA gene sequence of strain J211 assigned to Burkholderia arboris. Culture filtrates B. arboris J211 exhibited anematicidal activity against the second-stage juveniles (J2s) of M. incognita, with a 96.6% mortality after 24 h exposure. Inoculation of J211 in tobacco roots significantly reduced the root galling caused by M. incognita, both in pot and field trials. Meanwhile, plant growth-promoting (PGP) traits results showed that J211 had outstanding IAA-producing activity, and the IAA production reached 66.60 mg L^-1. In the field study, B. arboris J211 also promoted tobacco growth and increase flue-cured tobacco yield by 8.7-24.3%. Overall, B. arboris J211 as a high-yielding IAA nematicidal strain effectively controlled M. incognita and improved tobacco yield making it a promising alternative bionematocide.

Keywords: Meloidogyne incognita; biocontrol; nematode; plant growth-promoting rhizosphere (PGPR); tobacco.