Mowing Did Not Alleviate the Negative Effect of Nitrogen Addition on the Arbuscular Mycorrhizal Fungal Community in a Temperate Meadow Grassland

Siqi Qin; Guojiao Yang; Yang Zhang; Meixia Song; Lu Sun; Yangzhe Cui; Jibin Dong; Ning Wang; Xiao Liu; Peiming Zheng; Renqing Wang

doi:10.3389/fpls.2022.917645

Mowing Did Not Alleviate the Negative Effect of Nitrogen Addition on the Arbuscular Mycorrhizal Fungal Community in a Temperate Meadow Grassland

Front Plant Sci. 2022 Jun 9:13:917645. doi: 10.3389/fpls.2022.917645. eCollection 2022.

Authors

Siqi Qin^{1

2

3}, Guojiao Yang^{1

4}, Yang Zhang^{1

2

3}, Meixia Song^{1

2

3}, Lu Sun^{1

2

3}, Yangzhe Cui^{1

2

3}, Jibin Dong^{1

2

3}, Ning Wang^{1

2

3}, Xiao Liu^{1

2

3}, Peiming Zheng^{1

2

3}, Renqing Wang^{1

2

3}

Affiliations

¹ School of Life Sciences, Institute of Ecology and Biodiversity, Shandong University, Jinan, China.
² Shandong Provincial Engineering and Technology Research Center for Vegetation Ecology, Shandong University, Jinan, China.
³ Qingdao Forest Ecology Research Station of National Forestry and Grassland Administration, Shandong University, Jinan, China.
⁴ College of Ecology and Environment, Hainan University, Haikou, China.

Abstract

As nitrogen deposition intensifies under global climate change, understanding the responses of arbuscular mycorrhizal (AM) fungi to nitrogen deposition and the associated mechanisms are critical for terrestrial ecosystems. In this study, the effects of nitrogen addition and mowing on AM fungal communities in soil and mixed roots were investigated in an Inner Mongolia grassland. The results showed that nitrogen addition reduced the α-diversity of AM fungi in soil rather than that of root. Besides, nitrogen addition altered the composition of AM fungal community in soil. Soil pH and inorganic nitrogen content were the main causes of changes in AM fungal communities affected by nitrogen addition. Mowing and the interaction of nitrogen addition and mowing had no significant effect on AM fungal community diversity. In contrast, while mowing may reduce the negative effects of nitrogen addition on the richness and diversity of plants by alleviating light limitation, it could not do so with the negative effects on AM fungal communities. Furthermore, AM fungal communities clustered phylogenetically in all treatments in both soil and roots, indicating that environmental filtering was the main driving force for AM fungal community assembly. Our results highlight the different responses of AM fungi in the soil and roots of a grassland ecosystem to nitrogen addition and mowing. The study will improve our understanding of the effects of nitrogen deposition on the function of ecosystem.

Keywords: arbuscular mycorrhizal fungi; community composition; diversity; environmental filtering; nitrogen addition.