Titration and neutralizing antibody quantification by focus forming assay for Powassan virus

STAR Protoc. 2022 Jun 17;3(3):101473. doi: 10.1016/j.xpro.2022.101473. eCollection 2022 Sep 16.

Abstract

The development of high-throughput assays measuring Powassan virus (POWV) lineage I and II represents an important step in virological and immunological studies. By adapting focus-forming assays previously optimized for West Nile virus and Zika virus, this protocol is able to determine viral load, evaluate antivirals, and measure neutralizing antibodies. Although limited by its requirement of a detection antibody, this protocol includes a rapid and high-throughput assay for measuring viral titer. By utilizing a baby hamster kidney cell line and a 96-well plate format, this protocol allows for more sensitivity in the detection of POWV lineage I. For complete details on the use and execution of this protocol, please refer to Stone et al. (2022).

Keywords: Antibody; Cell-based Assays; Microbiology.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antibodies, Neutralizing
  • Cricetinae
  • Encephalitis Viruses, Tick-Borne*
  • Viral Load
  • West Nile virus*
  • Zika Virus Infection*
  • Zika Virus*

Substances

  • Antibodies, Neutralizing